Construct: ORF ccsbBroad304_05135
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011918.1_s304c1, BRDN0000461362
- Derived from:
- ccsbBroadEn_05135
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PARP15 (165631)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05135
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 165631 | PARP15 | poly(ADP-ribose) polymerase... | NM_152615.2 | 100% | 100% | |
| 2 | human | 165631 | PARP15 | poly(ADP-ribose) polymerase... | XM_011512478.3 | 95.4% | 94.8% | (many diffs) |
| 3 | human | 165631 | PARP15 | poly(ADP-ribose) polymerase... | XM_011512479.3 | 95.4% | 94.8% | (many diffs) |
| 4 | human | 165631 | PARP15 | poly(ADP-ribose) polymerase... | XM_017005792.2 | 95.4% | 94.8% | (many diffs) |
| 5 | human | 165631 | PARP15 | poly(ADP-ribose) polymerase... | NM_001308321.1 | 84.4% | 84.4% | 529_530ins207 |
| 6 | human | 165631 | PARP15 | poly(ADP-ribose) polymerase... | XM_011512477.3 | 74.5% | 65.4% | (many diffs) |
| 7 | human | 165631 | PARP15 | poly(ADP-ribose) polymerase... | XM_005247160.4 | 72.5% | 70.8% | (many diffs) |
| 8 | human | 165631 | PARP15 | poly(ADP-ribose) polymerase... | XM_011512475.3 | 68.8% | 66.2% | (many diffs) |
| 9 | human | 165631 | PARP15 | poly(ADP-ribose) polymerase... | XM_011512476.3 | 66.2% | 60.1% | (many diffs) |
| 10 | human | 165631 | PARP15 | poly(ADP-ribose) polymerase... | NM_001113523.3 | 64.8% | 62.3% | (many diffs) |
| 11 | human | 165631 | PARP15 | poly(ADP-ribose) polymerase... | NM_001308320.2 | 62.2% | 53.6% | (many diffs) |
| 12 | human | 165631 | PARP15 | poly(ADP-ribose) polymerase... | XM_017005791.2 | 61% | 59.4% | (many diffs) |
| 13 | human | 165631 | PARP15 | poly(ADP-ribose) polymerase... | XM_005247159.4 | 54.6% | 52.2% | (many diffs) |
| 14 | human | 165631 | PARP15 | poly(ADP-ribose) polymerase... | XM_011512480.3 | 17.2% | 14.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1401
- ORF length:
- 1332
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gctccaaaga attggattaa tatttttaca caatattgtt gtagtcagta 121 actgtttcta tttccaggca tttttagatg aattcactaa ctggtcaaga ataaatccca 181 acaaggccag gattcccatg gcaggagata cccaaggtgt ggtcgggact gtctctaagc 241 cttgtttcac agcatatgaa atgaaaatcg gtgcaattac ttttcaggtt gctactggag 301 atatagccac tgaacaggta gatgttattg taaactcaac agcaaggaca tttaatcgga 361 aatcaggtgt gtcaagagct attttagaag gtgctggaca agctgtggaa agtgaatgtg 421 ctgtactagc tgcacagcct cacagagatt ttataattac accaggtgga tgcttaaagt 481 gcaaaataat aattcatgtt cctgggggaa aagatgtcag gaaaacggtc accagtgttc 541 tagaagagtg tgaacagagg aagtacacat cggtttccct tccagccatt ggaacaggaa 601 atgccggaaa aaaccctatc acagttgctg ataacataat cgatgctatt gtagacttct 661 catcacaaca ttccacccca tcattaaaaa cagttaaagt tgtcattttt caacctgagc 721 tgctaaatat attctacgac agcatgaaaa aaagagacct ctctgcatca ctgaactttc 781 agtccacatt ctccatgact acatgtaatc ttcctgaaca ctggactgac atgaatcatc 841 agctgttttg catggtccag ctagagccag gacaatcaga atataatacc ataaaggaca 901 agttcacccg aacttgttct tcctacgcaa tagagaagat tgagaggata cagaatgcat 961 ttctctggca gagctaccag gtaaagaaaa ggcaaatgga tatcaagaat gaccataaga 1021 ataatgagag actcctcttc catgggacag atgcagactc agtgccatat gtcaatcagc 1081 acggctttaa tagaagttgt gctgggaaaa atgctgtatc ctatggaaaa ggaacctatt 1141 ttgctgtgga tgccagttat tctgccaagg acacctactc caagccagac agcaatggga 1201 gaaagcacat gtacgttgtg cgagtactta ctggagtctt cacaaaggga cgtgcaggat 1261 tagtcacccc tccacccaag aatcctcaca atcccacaga tctctttgac tcagtgacaa 1321 acaatacacg atctccaaag ctatttgtgg tattctttga taatcaggct tacccagaat 1381 atctcataac tttcacggct ttgccaactt tcttgtacaa agtggttggt aagcctatcc 1441 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 1501 ttaagtcgac aatcaacctc tggatta