Construct: ORF ccsbBroad304_05150
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000418.1_s304c1, BRDN0000403818
- Derived from:
- ccsbBroadEn_05150
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM47B (170062)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05150
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 170062 | FAM47B | family with sequence simila... | NM_152631.3 | 100% | 100% | |
| 2 | human | 158724 | FAM47A | family with sequence simila... | NM_203408.3 | 70.5% | 57% | (many diffs) |
| 3 | human | 442444 | FAM47C | family with sequence simila... | NM_001013736.2 | 57.2% | 49.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2001
- ORF length:
- 1935
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg ggaccggagg ccacaggacc ggccaaggtc ccaaggcatg gactccaagc 121 cctggtactg tgacaaaccg ccttccaagt acttcgcgaa gcgcaagcac aggcgcctga 181 ggttcccgcc tgtggacacc cagaactggg tatttgtgac ggagggcatg gacgacttcc 241 gctacgcctg tcagtctcct gaagatacgc ttgtttgtcg ccgtgacgag tttttactcc 301 ccaaaatatc tctcagaggt ccccaagctg accgcaaaag caggaagaaa aagctgctca 361 agaaagcggc cctattttcc gagctctcgc cagtacagcc agcacggaag gcgttcgtag 421 aggaagtgga agcccagctg atgaccaagc atcccttggc catgtacccc aatctgggaa 481 aagatatgcc tccagatctc ctactacagg tgctgaaaca gctggatccc gagaggaagc 541 tggaggacgc ttgggctcgt tgtgaggccc gggagaagac aaccgaggta cccaccgagt 601 ctggtaaata tccctgtggg gaatcctgcc cgcggcctcc cgagactccg gtgtcccgtc 661 tccgtcctca gcttcccaag actccggtgt ccagtcgccg cccagagcct cccaagactc 721 gggtgtccag tctccgccca gagcctccca agactcgggt gtccagtctc cacccggaac 781 ctccagagac tcgcgcatct catctccgcg tggatcctcc cgagactgga gtgtcccatc 841 tctgcccaga gcctcccaag actctggtgt ccagtgtcca cccagagcct cctgatactg 901 gagcgtccca tctctgcccg gagcctcccg agactcgcgt atctcatctc cacccggagc 961 ctcctgagac tggagtgtcc catctccgcc cagagccttc caagactcag gtgtccagtc 1021 tctgcccgga gcctcccgag gctggagtgt cccatctctg cctggaacct cccaacactc 1081 atcgggtgtc cagtttccta ctacaggtgc tgaaactgga ttctgagaag aagctggaag 1141 acgcacgggc tcgttgtgag ggccaggaga tgacaaccga ggaactcacc aagcctggta 1201 aataccattt ttgggaatcc tgtccgcggc cttttgagag tcggatgccc catctccgcc 1261 tggtgcttcc cataactcgt cgaatggcca gtctctgcct gaagcctccc aagactcgtc 1321 gggtgtccag tctctgcccg gagcctacca agaccggagc gtcccatcta aaagaactgt 1381 ttcaggaaga tacaccaagc acaatggagt gtgtttctga ctctcttcaa cgtagacaca 1441 catcgagaaa actccgtgac ttcaagtggg ctggagacct aggagttaat gaagaatcca 1501 tcagcagtct gtttgacttt acccctgagt gcagaacaac cgatcaagac caaaagatta 1561 agaaggcaaa cgagtgtgct tcaaggctga tgtacggcat ggagctagac gacatggatg 1621 aggtcgaatt cttacggata aaatactggg acaggagacg ccgggcggca ccgcattctt 1681 atagtgcaca gcgtgggagg ataaggtatg gaccatggta cttcgagcct aagttgggga 1741 aaaagctaag aagtgatgaa cctttgattg accccaagcc cgtacttgaa aagcctgatg 1801 aacccgacat tcttgacggt ctttatggac caattgcctt taaggatttc attctaagca 1861 agggctacag aatgcctggc gtcattgaaa agctgtttgc caagaaggga tggacttacg 1921 actctgttaa gactcctatt caacgtgcag tgcaagttta caagtacaaa gaagacgtca 1981 cagatgcatc aaaagaagat tacccaactt tcttgtacaa agtggttggt aagcctatcc 2041 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 2101 ttaagtcgac aatcaacctc tggatta