Construct: ORF ccsbBroad304_05176

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005947.1_s304c1, BRDN0000409397
Derived from:
ccsbBroadEn_05176
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MLKL (197259)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_05176
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 197259 MLKL mixed lineage kinase domain... NM_001142497.3 100% 100%
2 human 197259 MLKL mixed lineage kinase domain... XM_011522936.3 76.2% 62.8% (many diffs)
3 human 197259 MLKL mixed lineage kinase domain... NM_152649.4 52.4% 52.8% 536_1190del;1239_1240ins31
4 human 197259 MLKL mixed lineage kinase domain... XM_005255834.4 52.4% 52.8% 536_1190del;1239_1240ins31
5 human 197259 MLKL mixed lineage kinase domain... XR_001751863.2 33.1% (many diffs)
6 human 197259 MLKL mixed lineage kinase domain... XR_001751862.2 31.9% (many diffs)
7 human 197259 MLKL mixed lineage kinase domain... XR_429715.4 31.4% 1_412del;948_1602del;1857_2508del
8 human 197259 MLKL mixed lineage kinase domain... XR_001751861.2 31.3% (many diffs)
9 human 197259 MLKL mixed lineage kinase domain... XR_001751860.2 31.2% (many diffs)
10 human 197259 MLKL mixed lineage kinase domain... XR_001751859.2 30% (many diffs)
11 human 197259 MLKL mixed lineage kinase domain... XR_001751858.2 29.7% (many diffs)
12 human 197259 MLKL mixed lineage kinase domain... XR_002957791.1 28.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
855
ORF length:
789
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga aaatttgaag catattatca cccttggcca ggtcatccac aaacggtgtg
121aagagatgaa atactgcaag aaacagtgcc ggcgcctggg ccaccgcgtc ctcggcctga
181tcaagcctct ggagatgctc caggaccaag gaaagaggag cgtgccctct gagaagttaa
241ccacagccat gaaccgcttc aaggctgccc tggaggaggc taatggggag atagaaaagt
301tcagcaatag atccaatatc tgcaggtttc taacagcaag ccaggacaaa atactcttca
361aggacgtgaa caggaagctg agtgatgtct ggaaggagct ctcgctgtta cttcaggttg
421agcaacgcat gcctgtttca cccataagcc aaggagcgtc ctgggcacag gaagatcagc
481aggatgcaga cgaagacagg cgagctttcc agatgctaag aagagataat gaaaaaatag
541aagcttcact gagacgatta gaaatcaaca tgaaagaaat caaggaaact ttgaggcagt
601ctttggaatc gtcctctggg aaatcgccac tggagatatc ccgtttcaag gtgaagaatg
661tgaagactgg ctcagccagt ggctgtaatt ctgagaagat ccgcaagctg gtggctgtga
721agcggcagca ggagccactg ggtgaagact gcccttcaga gctgcgggag atcattgatg
781agtgccgggc ccatgatccc tctgtgcggc cctctgtgga tgaaatctta aagaaactct
841ccaccttttc taagtaccca acttccttgt acaaagtggt tggtaagcct atccctaacc
901ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
961cgacaatcaa cctctggatt a

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