Transcript: Human XR_001751863.2

PREDICTED: Homo sapiens mixed lineage kinase domain like pseudokinase (MLKL), transcript variant X10, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MLKL (197259)
Length:
2259
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001751863.2
NBCI Gene record:
MLKL (197259)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_001751863.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000003224 TCCCGTTTCAAGGCTGTAATT pLKO.1 1423 3UTR 100% 13.200 18.480 N MLKL n/a
2 TRCN0000003227 CCTTCGGCATTGGGTTATCTA pLKO.1 1689 3UTR 100% 5.625 7.875 N MLKL n/a
3 TRCN0000297257 CCTTCGGCATTGGGTTATCTA pLKO_005 1689 3UTR 100% 5.625 7.875 N MLKL n/a
4 TRCN0000003225 GCGTATATTTGGGATTTGCAT pLKO.1 1201 3UTR 100% 3.000 4.200 N MLKL n/a
5 TRCN0000196741 GAGTCAAATCTACAGCATATC pLKO.1 1303 3UTR 100% 10.800 8.640 N MLKL n/a
6 TRCN0000003226 CCCAACATCCTGCGTATATTT pLKO.1 1190 3UTR 100% 15.000 10.500 N MLKL n/a
7 TRCN0000279728 CCCAACATCCTGCGTATATTT pLKO_005 1190 3UTR 100% 15.000 10.500 N MLKL n/a
8 TRCN0000194888 CCTCTGTGGATGAAATCTTAA pLKO.1 1564 3UTR 100% 13.200 9.240 N MLKL n/a
9 TRCN0000196317 GAAGCTTCACTGAGACGATTA pLKO.1 887 3UTR 100% 10.800 7.560 N MLKL n/a
10 TRCN0000279794 GAAGCTTCACTGAGACGATTA pLKO_005 887 3UTR 100% 10.800 7.560 N MLKL n/a
11 TRCN0000003228 AGAGATGAAATACTGCAAGAA pLKO.1 469 3UTR 100% 4.950 3.465 N MLKL n/a
12 TRCN0000196761 GTGACTTGATTTGATCAATAG pLKO.1 1944 3UTR 100% 10.800 6.480 N MLKL n/a
13 TRCN0000194846 CCTCTGACAGTAACTTTGATA pLKO.1 1807 3UTR 100% 5.625 3.375 N MLKL n/a
14 TRCN0000279786 CCTCTGACAGTAACTTTGATA pLKO_005 1807 3UTR 100% 5.625 3.375 N MLKL n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001751863.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05176 pDONR223 100% 33.1% None (many diffs) n/a
2 ccsbBroad304_05176 pLX_304 0% 33.1% V5 (many diffs) n/a
3 TRCN0000481431 AAGATGTTGTCGGCGTGGTTCGCT pLX_317 66.1% 33.1% V5 (many diffs) n/a
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