Construct: ORF ccsbBroad304_05181
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008293.1_s304c1, BRDN0000412117
- Derived from:
- ccsbBroadEn_05181
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LCE4A (199834)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05181
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 199834 | LCE4A | late cornified envelope 4A | NM_178356.3 | 100% | 100% | |
| 2 | human | 254910 | LCE5A | late cornified envelope 5A | NM_178438.5 | 70.9% | 63.6% | (many diffs) |
| 3 | human | 353133 | LCE1C | late cornified envelope 1C | NM_178351.4 | 65.1% | 60.1% | (many diffs) |
| 4 | human | 353132 | LCE1B | late cornified envelope 1B | NM_178349.2 | 62.5% | 60.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 366
- ORF length:
- 297
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcctgccag cagaaccaac agcagtgcca gccccctccc aagtgtccta 121 tccccaagta tcccccaaaa tgtccctcaa agtgtgcatc ctcatgccca cctccaatct 181 cttcctgctg tggctccagc tctgggggct gtggttgctg cagctctgag ggaggtggct 241 gctgcctgag ccaccacaga caccataggt cccactgcca cagacccaag agctccaatt 301 gctatggcag tggcagtggc cagcagtctg ggggttctgg ctgctgctct ggagggggct 361 gttgcttgcc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 421 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 481 acctctggat ta