Construct: ORF ccsbBroad304_05225
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011322.1_s304c1, BRDN0000401107
- Derived from:
- ccsbBroadEn_05225
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MED19 (219541)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05225
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 219541 | MED19 | mediator complex subunit 19 | NM_153450.4 | 100% | 100% | |
| 2 | human | 219541 | MED19 | mediator complex subunit 19 | NM_001317078.1 | 73.6% | 73.5% | (many diffs) |
| 3 | human | 219541 | MED19 | mediator complex subunit 19 | NR_157587.1 | 43.4% | 1_36del;619_1339del | |
| 4 | mouse | 381379 | Med19 | mediator complex subunit 19 | NM_025885.3 | 68.2% | 73.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 648
- ORF length:
- 582
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga gaatttcacg gcactgtttg gggctcaggc tgacccacca ccgcccccaa 121 ccgcactcgg cttcggacca ggaaagcctc cacccccgcc accgcctcct gcgggcgggg 181 gacccggcac ggccccgcct cccaccgcgg ccacggctcc tcctggcgcg gacaagtcag 241 gagctggctg tggccctttt tacctcatga gggaactgcc aggtagcaca gagctgacag 301 gcagcacgaa tctgatcaca cactacaact tggaacaagc ctataataaa ttctgtggga 361 agaaggtgaa ggagaagcta agtaacttcc tgcctgacct gccagggatg attgatctgc 421 ctggttccca tgataacagc agcctccgct ctctcattga gaagccccct attctcagta 481 gctctttcaa tcctatcaca gggaccatgc tggccggctt ccgcctccac actggcccgt 541 tgccggagca gtgtcgtctg atgcatattc agcctcccaa gaagaagaat aagcacaagc 601 acaaacagag ccgtacccag gatcctgtcc ccccaggtaa acccagttat ccaactttct 661 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 721 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta