Construct: ORF ccsbBroad304_05389
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013964.1_s304c1, BRDN0000460607
- Derived from:
- ccsbBroadEn_05389
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IAH1 (285148)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05389
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285148 | IAH1 | isoamyl acetate hydrolyzing... | NM_001039613.3 | 100% | 100% | |
| 2 | human | 285148 | IAH1 | isoamyl acetate hydrolyzing... | NM_001320858.2 | 89.6% | 89.5% | 0_1ins75;2_3delTGinsCC |
| 3 | human | 285148 | IAH1 | isoamyl acetate hydrolyzing... | XM_024452813.1 | 79.1% | 72.7% | (many diffs) |
| 4 | human | 285148 | IAH1 | isoamyl acetate hydrolyzing... | NM_001320859.2 | 54.4% | 54.4% | 0_1ins339 |
| 5 | human | 285148 | IAH1 | isoamyl acetate hydrolyzing... | NM_001320860.2 | 54.4% | 54.4% | 0_1ins339 |
| 6 | human | 285148 | IAH1 | isoamyl acetate hydrolyzing... | NM_001320863.2 | 54.4% | 54.4% | 0_1ins339 |
| 7 | human | 285148 | IAH1 | isoamyl acetate hydrolyzing... | XR_001738723.1 | 47.5% | 1_3delTCC;81_82ins53;695_1401del | |
| 8 | human | 285148 | IAH1 | isoamyl acetate hydrolyzing... | XR_426950.2 | 25.6% | 1_35del;780_2901del | |
| 9 | human | 285148 | IAH1 | isoamyl acetate hydrolyzing... | XR_001738722.1 | 25.5% | 1_3delTCC;81_82ins53;695_2653del | |
| 10 | human | 285148 | IAH1 | isoamyl acetate hydrolyzing... | XR_002959280.1 | 20.3% | (many diffs) | |
| 11 | human | 285148 | IAH1 | isoamyl acetate hydrolyzing... | XR_001738718.1 | 18.2% | 1_35del;780_4084del | |
| 12 | human | 285148 | IAH1 | isoamyl acetate hydrolyzing... | XR_426953.3 | 17% | 1_3delTCC;81_82ins53;695_3999del | |
| 13 | human | 285148 | IAH1 | isoamyl acetate hydrolyzing... | XR_002959282.1 | 16% | 1_35del;780_4644del | |
| 14 | human | 285148 | IAH1 | isoamyl acetate hydrolyzing... | XR_001738721.1 | 15.2% | 1_35del;780_4865del | |
| 15 | human | 285148 | IAH1 | isoamyl acetate hydrolyzing... | XR_001738724.1 | 14.2% | 1_3delTCC;81_82ins53;695_4780del | |
| 16 | human | 285148 | IAH1 | isoamyl acetate hydrolyzing... | XR_002959281.1 | 12.4% | (many diffs) | |
| 17 | human | 285148 | IAH1 | isoamyl acetate hydrolyzing... | XR_002959279.1 | 6.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 813
- ORF length:
- 744
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgctgtgc gaggccgcgg gctgcgggag tgccctgctc tggcctcgct 121 tgttgctctt cggggactcc atcacccagt tttccttcca gcagggtgga tggggagcat 181 cgctggctga caggctggtc agaaaatgtg atgttctgaa tcgtggattt tcaggttaca 241 ataccaggtg ggccaaaatt atccttccaa gattaatcag gaaaggaaac agtttggaca 301 tcccagtagc agttacaatt ttctttgggg ccaatgacag tgcactaaaa gatgagaatc 361 ccaagcagca cattcccctg gaggagtacg ctgcgaacct aaagagcatg gtgcagtacc 421 tgaagtccgt ggacatccct gagaatcgag tcattctcat cacgccgacc ccactttgtg 481 aaacagcctg ggaagaacag tgcatcatac aaggttgcaa actaaatcgc ctgaactctg 541 ttgttggtga atatgccaat gcgtgtttac aagtggccca agactgtggg actgacgtac 601 ttgacctgtg gaccctgatg caggacagcc aggacttctc atcttattta tcagatggac 661 tacatttgtc tccaaagggg aatgaatttt tgttctcgca tctctggcct ttgatagaga 721 aaaaggtctc ttctctacct ttgctgcttc cttactggcg ggatgtagca gaagcaaaac 781 ctgaattaag tctgctggga gatggagacc atttgccaac tttcttgtac aaagtggttg 841 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 901 accggtggcg cgttaagtcg acaatcaacc tctggatta