Construct: ORF ccsbBroad304_05429
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018296.1_s304c1, BRDN0000401364
- Derived from:
- ccsbBroadEn_05429
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRTAP19-1 (337882)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05429
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 337882 | KRTAP19-1 | keratin associated protein ... | NM_181607.3 | 100% | 100% | |
| 2 | human | 337970 | KRTAP19-3 | keratin associated protein ... | NM_181609.4 | 83.7% | 83.3% | (many diffs) |
| 3 | human | 337972 | KRTAP19-5 | keratin associated protein ... | NM_181611.3 | 70% | 63.3% | (many diffs) |
| 4 | human | 337974 | KRTAP19-7 | keratin associated protein ... | NM_181614.2 | 60.3% | 54.4% | (many diffs) |
| 5 | human | 337973 | KRTAP19-6 | keratin associated protein ... | NM_181612.3 | 56.2% | 53.3% | (many diffs) |
| 6 | human | 337973 | KRTAP19-6 | keratin associated protein ... | NM_001303120.1 | 43.3% | 40.5% | (many diffs) |
| 7 | mouse | 105246181 | Gm41515 | predicted gene, 41515 | XM_011246170.1 | 78.5% | 74.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 339
- ORF length:
- 270
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gagtcactac ggcagctact acggaggcct gggctacagc tgtggaggct 121 tcggtggcct gggctatggc tatggctgtg gatgtggcag cttctgcaga cggggttctg 181 gctgtggcta tggaggctac ggatatggct ctggctttgg aagctacgga tatggctctg 241 gctttggagg ctacggatat ggctctggct ttggaggcta tggatatggc tgctgccgcc 301 catcgtacaa tggaggatac ggattctctg gcttttattt gccaactttc ttgtacaaag 361 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 421 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta