Construct: ORF ccsbBroad304_05446
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010223.1_s304c1, BRDN0000460540
- Derived from:
- ccsbBroadEn_05446
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNASE10 (338879)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05446
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 338879 | RNASE10 | ribonuclease A family membe... | NM_001012975.1 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 717
- ORF length:
- 648
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagctgaat ctggtgcaga tctttttcat gttgctgatg ctgctgctgg 121 gcctggggat gggcctgggg ttgggacttc atatggctac agcagtcttg gaggagagtg 181 atcaaccgct caatgaattt tggtccagtg actcacagga caaagctgag gccactgagg 241 agggagacgg cacccaaacc acagaaacgc tggtgcttag caacaaagaa gtggtgcaac 301 ctggctggcc agaagatccc atcctcggtg aagatgaggt tgggggtaac aagatgctca 361 gagcctcagc tctctttcag agcaacaaag actatcttag gcttgaccag acagatagag 421 aatgcaatga tatgatggca cacaagatga aggagcccag tcagagttgc atagcccagt 481 atgcattcat ccatgaggat ctaaacacag tcaaagctgt ctgtaacagt cctgtcattg 541 cctgtgagct caagggggga aaatgtcaca aaagctcccg accttttgat ttgacattgt 601 gcgagctgtc ccaaccagac caggtcactc ctaactgcaa ttacctaact tctgttataa 661 aaaagcacat tattataacc tgtaatgaca tgaagcgcca gttaccaact ggacaattgc 721 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 781 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 841 tta