Construct: ORF ccsbBroad304_05499
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015603.1_s304c1, BRDN0000411753
- Derived from:
- ccsbBroadEn_05499
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RSPH4A (345895)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05499
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 345895 | RSPH4A | radial spoke head component 4A | NM_001161664.1 | 100% | 100% | |
| 2 | human | 345895 | RSPH4A | radial spoke head component 4A | XM_017010826.1 | 100% | 100% | |
| 3 | human | 345895 | RSPH4A | radial spoke head component 4A | NM_001010892.3 | 83.7% | 76.8% | 1661_1796del;1937_2148del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1869
- ORF length:
- 1800
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaggactca acctccccga agcaagaaaa agaaaaccaa gaagaactag 121 gggaaacaag gcggccatgg gaaggaaaga cagcagcttc tccccaatat tctgagcctg 181 agtcgtctga gcccttggag gcgaagcagg ggccagaaac tggacgccag tcccgaagca 241 gccgtccttg gagcccgcag tctagagcca agacgcctct gggtggcccc gcgggaccag 301 aaacatcatc acctgctcct gtctctccgc gggagccctc ttcctctcct tctcccctgg 361 ctccggccag acaagacctc gcggcaccac ctcagtcgga caggaccacg agtgtgattc 421 ctgaagctgg gacaccttat cctgatcctt tggaacaatc atctgataaa agagaatcaa 481 ctcctcatca cacaagccag tcagaaggaa acacctttca acagtctcag caacccaaac 541 cccacctgtg tggacgaagg gacgtgagct ataacaacgc taaacagaaa gagctgagat 601 ttgacgtttt tcaggaggaa gactcaaaca gtgactatga tttacagcag ccggcgcctg 661 ggggctctga agtggccccc agcatgcttg agatcaccat tcagaatgct aaggcttacc 721 tgctgaagac tagcagcaat tcgggcttta atctatatga tcatctttct aatatgttga 781 ccaagatatt aaatgagcgt cctgaaaatg ctgttgacat ctttgaaaat attagccaag 841 atgtgaagat ggcacatttt agtaaaaaat ttgatgcact acaaaatgag aatgagttgc 901 ttccaacata tgaaatagca gaaaagcaaa aggctctttt tctccaggga catttggaag 961 gagttgacca agaattggaa gatgaaatag cagaaaacgc tcttccaaat gtaatggagt 1021 cagcttttta ttttgaacaa gctggagttg gtttgggcac agatgagaca taccgcatat 1081 ttcttgccct caagcagctt actgataccc acccaatcca aagatgccgc ttctggggaa 1141 agatcttggg tctggaaatg aattatattg tagctgaagt ggaatttcgt gagggggaag 1201 atgaagagga agtggaagag gaagatgtag ctgaagagag ggacaatgga gaaagtgaag 1261 ctcatgaaga tgaggaagat gaattaccaa agtcctttta caaggcccca caggctatac 1321 caaaagaaga aagtagaaca ggtgccaaca aatatgtcta ttttgtttgc aatgaaccag 1381 gaagaccatg ggtgaagtta ccaccagtta tacctgcaca aattgttatt gcaagaaaaa 1441 tcaagaaatt tttcactggg cgattggatg ctcccatcat aagctaccca cctttcccag 1501 gaaatgagag taattattta cgagcacaaa ttgcccgaat ttcagcagga acccacgtca 1561 gtcctctagg attttatcag tttggtgaag aggaaggaga ggaggaggaa gaggcagaag 1621 gtgggcgaaa tagctttgag gaaaaccctg attttgaagg catccaagtg attgatctag 1681 tagaatccct atccaattgg gttcatcatg tacagcatat tctctctcag agattcagaa 1741 tatacccccc tggacaacac ggttatcctc aaatctcatt ccacaatatg ctattgcagt 1801 ccttcaatcc aacctttggc ctggagcata tgccttctcc aatggcaaaa agtttgaaaa 1861 tttctacatt gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 1921 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 1981 tcaacctctg gatta