Construct: ORF ccsbBroad304_05502

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000138.1_s304c1, BRDN0000461788
Derived from:
ccsbBroadEn_05502
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MACC1 (346389)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_05502
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 346389 MACC1 MET transcriptional regulat... NM_182762.4 100% 100%
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2625
ORF length:
2556
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gctaatcact gaaagaaaac attttcggtc aggaagaatt gcacaaagta
121tgtctgaagc aaatttgatt gacatggaag ctggaaaact ctcaaaaagt tgcaatatta
181cagaatgcca ggacccagac ttgcttcaca attggccgga tgctttcacc cttcgtggta
241ataatgcttc caaagttgca aatccattct ggaatcaact gtctgcttct aacccatttt
301tggatgacat aactcaacta agaaataaca ggaagagaaa taatatttcc atcttaaagg
361aagatccttt tcttttctgt agagaaatag aaaatggaaa ttcttttgat tcctccggtg
421atgaacttga tgtgcatcag ttacttaggc agacttcctc aagaaattct ggaagatcta
481aaagtgtttc agaacttctg gacattttag acgacacagc acatgcccat cagagtatac
541ataactctga ccagatccta ctacacgact tagagtggct taaaaatgat cgggaggctt
601ataaaatggc ttggttaagt caacgccagc tggcccgctc ctgccttgat ttgaatacaa
661ttagtcagag ccctggatgg gcccagacac aacttgcgga ggtcaccata gcttgcaaag
721taaaccatca aggagggtca gtacaattac ctgaatcaga catcactgtt catgtgcccc
781aaggtcatgt ggctgtggga gaattccaag aggtgtctct aagggctttc cttgatccgc
841cacacatgct taaccatgat ctttcgtgca ctgtgagccc gttgttggaa atcatgttag
901gcaacctcaa tacaatggaa gcccttttgc tggagatgaa aattggggct gaagtaagaa
961aggatccttt cagccaagtc atgacagaaa tggtgtgttt acacagcttg ggtaaagaag
1021gcccttttaa agttttaagc aactgctaca tttataaaga caccatccaa gtcaagctaa
1081tcgacttgag tcaggtaatg tatctagtgg ttgctgcaca agctaaagct cttccgtcac
1141cagctgccac catttgggat tatatccaca aaaccacctc aattggaatt tatggaccca
1201aatatatcca tcccagtttt actgttgttt taacagtttg tggacacaat tatatgccag
1261gacagcttac aatttctgat attaagaagg gtggaaaaaa catatctcca gttgtgtttc
1321agctctgggg gaagcagtca tttttacttg acaagccaca agatttaagt atttctattt
1381tttcctgtga tcctgatttt gaagtaaaga cagaaggaga aaggaaagaa attaaacaaa
1441agcagttgga agcaggtgaa gtagttcatc aacaattttt attttcttta gttgagcaca
1501gagagatgca cttgtttgat ttttgtgttc aagtggagcc tcccaatggt gaaccagttg
1561cacagttctc tatcactact cctgatccaa ccccaaacct aaaaagactc tcgaatctgc
1621caggctattt gcagaagaag gaggaaatca agtctgctcc tttatcacca aaaattcttg
1681ttaaatatcc tacatttcaa gataaaacat tgaactttag caactatggg gtaaccctga
1741aggcagtgct aagacaaagc aagattgatt acttccttga atatttcaaa ggggacacaa
1801tagctctcct cggggaaggt aaggtaaaag ctattggtca gtccaaagtg aaagaatggt
1861atgtaggagt cctcagaggt aagattggac ttgtacactg caaaaatgtc aaggtgattt
1921caaaggagca agtaatgttt atgtcagata gtgtctttac aaccagaaat cttcttgaac
1981agattgtcct gcctttaaaa aaattgactt atatctactc agttgtatta accttggtgt
2041cagaaaaagt ttatgattgg aaagttttag ctgatgtcct gggttactca catctgtccc
2101tggaagattt tgatcaaatt caagcagaca aagaatcaga gaaagtttct tatgttataa
2161agaagttaaa ggaagattgc cacacagaga gaaatacaag gaagtttctg tatgaactta
2221ttgtggctct tctgaaaatg gattgccaag agttagtcgc acgtctcatc caagaagctg
2281ctgttctgac ttcagctgtc aagcttggaa aaggctggag ggaactagct gaaaagttag
2341tacgactcac aaagcaacaa atggaggcat atgaaattcc tcatcgagga aacactggag
2401atgttgctgt tgagatgatg tggaaacctg cctatgattt tctgtatacc tggagtgctc
2461actatggaaa taactacaga gatgtgttac aagaccttca gtcagctttg gacagaatga
2521aaaaccctgt gactaaacac tggagagaat taactggagt tttaatacta gtaaattctt
2581tggaggtttt gagagtaact gcattctcca cttctgagga agtattgcca actttcttgt
2641acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
2701gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

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