Construct: ORF ccsbBroad304_05577

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017302.1_s304c1, BRDN0000460638
Derived from:
ccsbBroadEn_05577
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SPDYE4 (388333)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_05577
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 388333 SPDYE4 speedy/RINGO cell cycle reg... NM_001128076.3 100% 100%
2 human 388333 SPDYE4 speedy/RINGO cell cycle reg... XM_005256633.4 100% 100%
3 human 100996746 SPDYE11 speedy/RINGO cell cycle reg... NM_001351349.1 72.2% 62.2% (many diffs)
4 human 102723849 SPDYE17 speedy/RINGO cell cycle reg... NM_001351351.1 72.2% 62.2% (many diffs)
5 human 100505767 SPDYE18 speedy/RINGO cell cycle reg... NM_001351348.1 69.9% 59.4% (many diffs)
6 human 285955 SPDYE1 speedy/RINGO cell cycle reg... XM_005249719.3 52.4% 45.5% (many diffs)
7 human 285955 SPDYE1 speedy/RINGO cell cycle reg... XM_017012015.1 52.4% 45.5% (many diffs)
8 human 441273 SPDYE2 speedy/RINGO cell cycle reg... NM_001031618.3 50.2% 41.7% (many diffs)
9 human 100310812 SPDYE2B speedy/RINGO cell cycle reg... NM_001166339.1 50.2% 41.7% (many diffs)
10 human 100310812 SPDYE2B speedy/RINGO cell cycle reg... XM_005250093.4 50.2% 41.7% (many diffs)
11 human 441273 SPDYE2 speedy/RINGO cell cycle reg... XM_017012223.1 50.2% 41.7% (many diffs)
12 human 442590 SPDYE5 speedy/RINGO cell cycle reg... NM_001306141.1 50% 42.2% (many diffs)
13 human 729597 SPDYE6 speedy/RINGO cell cycle reg... NM_001146210.4 50% 41.7% (many diffs)
14 human 728524 SPDYE8P speedy/RINGO cell cycle reg... NR_003664.2 20.4% (many diffs)
15 human 643862 SPDYE10P speedy/RINGO cell cycle reg... NR_146079.1 20.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
780
ORF length:
711
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggccagtggt caagcgcgcc ccccgtttga ggaggagagc ccccagccta
121gcacaacggt acggtccccc gaggtggtgg tggatgatga agtgccagga ccatcagccc
181cttggataga tcccagcccc cagcctcaat cccttggcct gaaaaggaag agcgaatggt
241cagacgaatc cgaggaggag ctggaggagg agctggagtt ggagcgcgcc cctgagcccg
301aggacacctg ggtggtggag acgctgtgtg ggctcaagat gaagctgaag cgaaagcgag
361catcctccgt gctccctgag caccacgagg ccttcaacag gctccttggg gatcctgtcg
421ttcaaaaatt cctggcctgg gacaaagacc tgagggtgtc agacaagtat ctcctggcta
481tggtcatagc gtattttagc cgtgccggcc tcttctcgtg gcaataccaa cgcattcatt
541tcttcctggc tctctacctg gccagtgaca tggaggagga caaccaggcc ccgaaacaag
601acatcttctc cttcctctac gggaagaact actcccagcg acccttgttc cataagcttc
661gataccagct cctctgttcc atgcgctgga ggacgtgggt ttccccagag gagatggagg
721agatccaggc ttatgaccca gagcactggg tgtgggcccg agatcgcacc ctcatttcct
781tgccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg
841attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct
901ggatta

Download FASTA (ORF) (Full)