Construct: ORF ccsbBroad304_05577
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017302.1_s304c1, BRDN0000460638
- Derived from:
- ccsbBroadEn_05577
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPDYE4 (388333)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05577
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 388333 | SPDYE4 | speedy/RINGO cell cycle reg... | NM_001128076.3 | 100% | 100% | |
| 2 | human | 388333 | SPDYE4 | speedy/RINGO cell cycle reg... | XM_005256633.4 | 100% | 100% | |
| 3 | human | 100996746 | SPDYE11 | speedy/RINGO cell cycle reg... | NM_001351349.1 | 72.2% | 62.2% | (many diffs) |
| 4 | human | 102723849 | SPDYE17 | speedy/RINGO cell cycle reg... | NM_001351351.1 | 72.2% | 62.2% | (many diffs) |
| 5 | human | 100505767 | SPDYE18 | speedy/RINGO cell cycle reg... | NM_001351348.1 | 69.9% | 59.4% | (many diffs) |
| 6 | human | 285955 | SPDYE1 | speedy/RINGO cell cycle reg... | XM_005249719.3 | 52.4% | 45.5% | (many diffs) |
| 7 | human | 285955 | SPDYE1 | speedy/RINGO cell cycle reg... | XM_017012015.1 | 52.4% | 45.5% | (many diffs) |
| 8 | human | 441273 | SPDYE2 | speedy/RINGO cell cycle reg... | NM_001031618.3 | 50.2% | 41.7% | (many diffs) |
| 9 | human | 100310812 | SPDYE2B | speedy/RINGO cell cycle reg... | NM_001166339.1 | 50.2% | 41.7% | (many diffs) |
| 10 | human | 100310812 | SPDYE2B | speedy/RINGO cell cycle reg... | XM_005250093.4 | 50.2% | 41.7% | (many diffs) |
| 11 | human | 441273 | SPDYE2 | speedy/RINGO cell cycle reg... | XM_017012223.1 | 50.2% | 41.7% | (many diffs) |
| 12 | human | 442590 | SPDYE5 | speedy/RINGO cell cycle reg... | NM_001306141.1 | 50% | 42.2% | (many diffs) |
| 13 | human | 729597 | SPDYE6 | speedy/RINGO cell cycle reg... | NM_001146210.4 | 50% | 41.7% | (many diffs) |
| 14 | human | 728524 | SPDYE8P | speedy/RINGO cell cycle reg... | NR_003664.2 | 20.4% | (many diffs) | |
| 15 | human | 643862 | SPDYE10P | speedy/RINGO cell cycle reg... | NR_146079.1 | 20.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 780
- ORF length:
- 711
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccagtggt caagcgcgcc ccccgtttga ggaggagagc ccccagccta 121 gcacaacggt acggtccccc gaggtggtgg tggatgatga agtgccagga ccatcagccc 181 cttggataga tcccagcccc cagcctcaat cccttggcct gaaaaggaag agcgaatggt 241 cagacgaatc cgaggaggag ctggaggagg agctggagtt ggagcgcgcc cctgagcccg 301 aggacacctg ggtggtggag acgctgtgtg ggctcaagat gaagctgaag cgaaagcgag 361 catcctccgt gctccctgag caccacgagg ccttcaacag gctccttggg gatcctgtcg 421 ttcaaaaatt cctggcctgg gacaaagacc tgagggtgtc agacaagtat ctcctggcta 481 tggtcatagc gtattttagc cgtgccggcc tcttctcgtg gcaataccaa cgcattcatt 541 tcttcctggc tctctacctg gccagtgaca tggaggagga caaccaggcc ccgaaacaag 601 acatcttctc cttcctctac gggaagaact actcccagcg acccttgttc cataagcttc 661 gataccagct cctctgttcc atgcgctgga ggacgtgggt ttccccagag gagatggagg 721 agatccaggc ttatgaccca gagcactggg tgtgggcccg agatcgcacc ctcatttcct 781 tgccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 841 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 901 ggatta