Construct: ORF ccsbBroad304_05680
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016406.1_s304c1, BRDN0000408439
- Derived from:
- ccsbBroadEn_05680
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RTL8B (441518)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05680
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 441518 | RTL8B | retrotransposon Gag like 8B | NM_001078173.2 | 100% | 100% | |
| 2 | human | 26071 | RTL8A | retrotransposon Gag like 8A | NM_001078172.1 | 96.4% | 96.4% | (many diffs) |
| 3 | human | 8933 | RTL8C | retrotransposon Gag like 8C | NM_001078171.2 | 94.3% | 92.9% | (many diffs) |
| 4 | human | 26071 | RTL8A | retrotransposon Gag like 8A | NM_001134321.2 | 64.5% | 53.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 408
- ORF length:
- 339
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaaggtcga gtgcagctga tgaaggccct cctggctcgg cccctccggc 121 ccgcggcgcg tcgctggagg aacccgattc cctttcccga gacgtttgat ggcgataccg 181 accggctccc ggagttcatc gtgcagacga gctcctacat gttcgtggac gagaacacgt 241 tctccaacga cgccctgaag gtgacgttcc tcatcacccg cctcacgggg cccgccctgc 301 agtgggtgat cccctacatc aagaaggaga gccccctcct cagtgattac cggggcttcc 361 tggctgagat gaagcgggtc tttggatggg aggaggacga ggacttcttg ccaactttct 421 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 481 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta