Construct: ORF ccsbBroad304_05693
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007432.1_s304c1, BRDN0000407755
- Derived from:
- ccsbBroadEn_05693
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COA5 (493753)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05693
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 493753 | COA5 | cytochrome c oxidase assemb... | NM_001008215.3 | 100% | 100% | |
| 2 | mouse | 76178 | Coa5 | cytochrome C oxidase assemb... | NM_198006.4 | 85.5% | 82.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 288
- ORF length:
- 222
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc taagtattat gaggacaagc cgcagggcgg cgcgtgcgcg ggcctgaagg 121 aggacctggg cgcgtgtctg ctgcagtcgg actgtgtggt ccaggaagga aaatcacctc 181 ggcagtgttt gaaggaagga tactgcaact ctttgaagta cgcatttttt gagtgtaaaa 241 gatcagtgtt ggataacagg gcaagattca gaggaagaaa aggatattgc ccaactttct 301 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 361 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta