Construct: ORF ccsbBroad304_05716
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014611.1_s304c1, BRDN0000460222
- Derived from:
- ccsbBroadEn_05716
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM25C (644054)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05716
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 644054 | FAM25C | family with sequence simila... | NM_001137548.3 | 100% | 100% | |
| 2 | human | 100133093 | FAM25G | family with sequence simila... | NM_001137549.1 | 100% | 100% | |
| 3 | human | 643161 | FAM25A | family with sequence simila... | NM_001146157.3 | 97.7% | 100% | (many diffs) |
| 4 | human | 100132929 | FAM25BP | family with sequence simila... | NR_104039.1 | 76.2% | 1_26del;90T>G;294_349del | |
| 5 | human | 643479 | FAM25E | family with sequence simila... | NM_001351234.1 | 67% | 66.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 336
- ORF length:
- 267
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gctgggaggc ctggggaagc tggctgccga gggcctggcc caccgcaccg 121 agaaggccac cgagggagcc attcatgccg tggaggaagt ggtgaaggag gtggtgggac 181 atgccaagga gactggagag aaagccattg ctgaagccat aaagaaagcc caggagtcag 241 gggacaaaaa gatgaaggaa atcaccgaga cagtgaccaa cacagtcaca aatgccatca 301 cccatgcagc agaaagtctg gacaaacttg gacagttgcc aactttcttg tacaaagtgg 361 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 421 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta