Construct: ORF ccsbBroad304_05718
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006930.1_s304c1, BRDN0000408881
- Derived from:
- ccsbBroadEn_05718
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYCE3 (644186)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05718
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 644186 | SYCE3 | synaptonemal complex centra... | NM_001123225.3 | 100% | 100% | |
| 2 | human | 644186 | SYCE3 | synaptonemal complex centra... | XM_024452261.1 | 100% | 100% | |
| 3 | mouse | 75459 | Syce3 | synaptonemal complex centra... | NM_001162880.1 | 87.1% | 89.7% | (many diffs) |
| 4 | mouse | 75459 | Syce3 | synaptonemal complex centra... | NM_001162882.1 | 87.1% | 89.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 333
- ORF length:
- 264
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatgatgct gaccctgagg aaagaaacta tgacaacatg ctgaaaatgc 121 tgtcagatct gaataaggac ttggaaaagc tattagaaga gatggagaaa atctcagtgc 181 aggcgacctg gatggcctat gacatggtgg tgatgcgcac caaccctacg ctggccgagt 241 ccatgcgtcg gctggaggat gccttcgtca actgcaagga ggagatggag aagaactggc 301 aagagctgct gcatgagacc aagcaaaggc tgttgccaac tttcttgtac aaagtggttg 361 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 421 accggtggcg cgttaagtcg acaatcaacc tctggatta