Construct: ORF ccsbBroad304_05776
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002075.1_s304c1, BRDN0000461025
- Derived from:
- ccsbBroadEn_05776
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADH1B (125)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05776
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 125 | ADH1B | alcohol dehydrogenase 1B (c... | NM_000668.6 | 99.5% | 99.4% | (many diffs) |
| 2 | human | 126 | ADH1C | alcohol dehydrogenase 1C (c... | NM_000669.5 | 95.5% | 94.4% | (many diffs) |
| 3 | human | 124 | ADH1A | alcohol dehydrogenase 1A (c... | NM_000667.4 | 94.8% | 93.3% | (many diffs) |
| 4 | human | 125 | ADH1B | alcohol dehydrogenase 1B (c... | NM_001286650.2 | 89% | 89% | (many diffs) |
| 5 | human | 126 | ADH1C | alcohol dehydrogenase 1C (c... | NR_133005.2 | 70.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1191
- ORF length:
- 1125
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag cacggcagga aaaataatca aatgcaaagc agctgtgcta tgggaggtaa 121 agaaaccctt ttccattgag gatgtggagg ttgcacctcc taaggcttat gaagttcgca 181 ttaagatggt ggctgtagga atctgtcgca cagatgacca cgtggttagt ggcaacctgg 241 tgacccccct tcctgtgatt ttaggccatg aggcagccgg catcgtggag agtgttggag 301 aaggggtgac tacagtcaaa ccaggtgata aagtcatccc gctctttact cctcagtgtg 361 gaaaatgcag agtttgtaaa aacccggaga gcaactactg cttgaaaaat gatctaggca 421 atcctcgggg gaccctgcag gatggcacca ggaggttcac ctgcaggggg aagcccattc 481 accacttcct tggcaccagc accttctccc agtacacggt ggtggatgag aatgcagtgg 541 ccaaaattga tgcagcctcg cccctggaga aagtctgcct cattggctgt ggattctcga 601 ctggttatgg gtctgcagtt aacgttgcca aggtcacccc aggctctacc tgtgctgtgt 661 ttggcctggg aggggtcggc ctatctgctg ttatgggctg taaagcagct ggagcagcca 721 gaatcattgc tgtggacatc aacaaggaca aatttgcaaa ggccaaagag ttgggtgcca 781 ctgaatgcat caaccctcaa gactacaaga aacccatcca ggaagtgcta aaggaaatga 841 ctgatggagg tgtggatttt tcgtttgaag tcatcggtcg gcttgacacc atgatggctt 901 ccctgttatg ttgtcatgag gcatgtggca caagcgtcat cgtaggggta cctcctgctt 961 cccagaacct ctcaataaac cctatgctgc tactgactgg acgcacctgg aagggggctg 1021 tttatggtgg ctttaagagt aaagaaggta tcccaaaact tgtggctgat tttatggcta 1081 agaagttttc actggatgcg ttaataaccc atgttttacc ttttgaaaaa ataaatgaag 1141 gatttgacct gcttcactct gggaaaagta tccgtaccgt cctgacgttt tgcccaactt 1201 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 1261 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta