Construct: ORF ccsbBroad304_05782

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003975.1_s304c1, BRDN0000403479
Derived from:
ccsbBroadEn_05782
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ADORA2A (135)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_05782
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 135 ADORA2A adenosine A2a receptor NM_000675.6 99.9% 100% 1083T>C
2 human 135 ADORA2A adenosine A2a receptor NM_001278497.1 99.9% 100% 1083T>C
3 human 135 ADORA2A adenosine A2a receptor NM_001278498.1 99.9% 100% 1083T>C
4 human 135 ADORA2A adenosine A2a receptor NM_001278499.2 99.9% 100% 1083T>C
5 human 135 ADORA2A adenosine A2a receptor NM_001278500.1 99.9% 100% 1083T>C
6 human 135 ADORA2A adenosine A2a receptor NR_103543.2 47.5% (many diffs)
7 human 135 ADORA2A adenosine A2a receptor NR_103544.2 47.4% (many diffs)
8 human 101730217 SPECC1L-ADORA2A SPECC1L-ADORA2A readthrough... NR_103546.1 19.6% 1_4179del;5262T>C;5416_6289del
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1302
ORF length:
1236
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcc catcatgggc tcctcggtgt acatcacggt ggagctggcc attgctgtgc
121tggccatcct gggcaatgtg ctggtgtgct gggccgtgtg gctcaacagc aacctgcaga
181acgtcaccaa ctactttgtg gtgtcactgg cggcggccga catcgcagtg ggtgtgctcg
241ccatcccctt tgccatcacc atcagcaccg ggttctgcgc tgcctgccac ggctgcctct
301tcattgcctg cttcgtcctg gtcctcacgc agagctccat cttcagtctc ctggccatcg
361ccattgaccg ctacattgcc atccgcatcc cgctccggta caatggcttg gtgaccggca
421cgagggctaa gggcatcatt gccatctgct gggtgctgtc gtttgccatc ggcctgactc
481ccatgctagg ttggaacaac tgcggtcagc caaaggaggg caagaaccac tcccagggct
541gcggggaggg ccaagtggcc tgtctctttg aggatgtggt ccccatgaac tacatggtgt
601acttcaactt ctttgcctgt gtgctggtgc ccctgctgct catgctgggt gtctatttgc
661ggatcttcct ggcggcgcga cgacagctga agcagatgga gagccagcct ctgccggggg
721agcgggcacg gtccacactg cagaaggagg tccatgctgc caagtcactg gccatcattg
781tggggctctt tgccctctgc tggctgcccc tacacatcat caactgcttc actttcttct
841gccccgactg cagccacgcc cctctctggc tcatgtacct ggccatcgtc ctctcccaca
901ccaattcggt tgtgaatccc ttcatctacg cctaccgtat ccgcgagttc cgccagacct
961tccgcaagat cattcgcagc cacgtcctga ggcagcaaga acctttcaag gcagctggca
1021ccagtgcccg ggtcttggca gctcatggca gtgacggaga gcaggtcagc ctccgtctca
1081acggccaccc gccaggagtg tgggccaacg gcagtgctcc ccaccctgag cggaggccca
1141atggctacgc cctggggctg gtgagtggag ggagtgccca agagtcccag gggaacacgg
1201gcctcccaga cgtggagctc cttagccatg agctcaaggg agtgtgccca gagccccctg
1261gcctagatga ccccctggcc caggatggag caggagtgtc ctgcccaact ttcttgtaca
1321aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
1381ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

Download FASTA (ORF) (Full)