Construct: ORF ccsbBroad304_05782
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003975.1_s304c1, BRDN0000403479
- Derived from:
- ccsbBroadEn_05782
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADORA2A (135)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05782
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 135 | ADORA2A | adenosine A2a receptor | NM_000675.6 | 99.9% | 100% | 1083T>C |
| 2 | human | 135 | ADORA2A | adenosine A2a receptor | NM_001278497.1 | 99.9% | 100% | 1083T>C |
| 3 | human | 135 | ADORA2A | adenosine A2a receptor | NM_001278498.1 | 99.9% | 100% | 1083T>C |
| 4 | human | 135 | ADORA2A | adenosine A2a receptor | NM_001278499.2 | 99.9% | 100% | 1083T>C |
| 5 | human | 135 | ADORA2A | adenosine A2a receptor | NM_001278500.1 | 99.9% | 100% | 1083T>C |
| 6 | human | 135 | ADORA2A | adenosine A2a receptor | NR_103543.2 | 47.5% | (many diffs) | |
| 7 | human | 135 | ADORA2A | adenosine A2a receptor | NR_103544.2 | 47.4% | (many diffs) | |
| 8 | human | 101730217 | SPECC1L-ADORA2A | SPECC1L-ADORA2A readthrough... | NR_103546.1 | 19.6% | 1_4179del;5262T>C;5416_6289del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1302
- ORF length:
- 1236
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc catcatgggc tcctcggtgt acatcacggt ggagctggcc attgctgtgc 121 tggccatcct gggcaatgtg ctggtgtgct gggccgtgtg gctcaacagc aacctgcaga 181 acgtcaccaa ctactttgtg gtgtcactgg cggcggccga catcgcagtg ggtgtgctcg 241 ccatcccctt tgccatcacc atcagcaccg ggttctgcgc tgcctgccac ggctgcctct 301 tcattgcctg cttcgtcctg gtcctcacgc agagctccat cttcagtctc ctggccatcg 361 ccattgaccg ctacattgcc atccgcatcc cgctccggta caatggcttg gtgaccggca 421 cgagggctaa gggcatcatt gccatctgct gggtgctgtc gtttgccatc ggcctgactc 481 ccatgctagg ttggaacaac tgcggtcagc caaaggaggg caagaaccac tcccagggct 541 gcggggaggg ccaagtggcc tgtctctttg aggatgtggt ccccatgaac tacatggtgt 601 acttcaactt ctttgcctgt gtgctggtgc ccctgctgct catgctgggt gtctatttgc 661 ggatcttcct ggcggcgcga cgacagctga agcagatgga gagccagcct ctgccggggg 721 agcgggcacg gtccacactg cagaaggagg tccatgctgc caagtcactg gccatcattg 781 tggggctctt tgccctctgc tggctgcccc tacacatcat caactgcttc actttcttct 841 gccccgactg cagccacgcc cctctctggc tcatgtacct ggccatcgtc ctctcccaca 901 ccaattcggt tgtgaatccc ttcatctacg cctaccgtat ccgcgagttc cgccagacct 961 tccgcaagat cattcgcagc cacgtcctga ggcagcaaga acctttcaag gcagctggca 1021 ccagtgcccg ggtcttggca gctcatggca gtgacggaga gcaggtcagc ctccgtctca 1081 acggccaccc gccaggagtg tgggccaacg gcagtgctcc ccaccctgag cggaggccca 1141 atggctacgc cctggggctg gtgagtggag ggagtgccca agagtcccag gggaacacgg 1201 gcctcccaga cgtggagctc cttagccatg agctcaaggg agtgtgccca gagccccctg 1261 gcctagatga ccccctggcc caggatggag caggagtgtc ctgcccaact ttcttgtaca 1321 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 1381 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta