Construct: ORF ccsbBroad304_05837
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014842.1_s304c1, BRDN0000398695
- Derived from:
- ccsbBroadEn_05837
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- APOC1 (341)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05837
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 341 | APOC1 | apolipoprotein C1 | NM_001321065.2 | 99.5% | 92.7% | 232A>T |
| 2 | human | 341 | APOC1 | apolipoprotein C1 | NM_001321066.2 | 99.5% | 92.7% | 232A>T |
| 3 | human | 341 | APOC1 | apolipoprotein C1 | NM_001645.5 | 99.5% | 92.7% | 232A>T |
| 4 | human | 342 | APOC1P1 | apolipoprotein C1 pseudogene 1 | NR_028414.1 | 35.1% | (many diffs) | |
| 5 | human | 342 | APOC1P1 | apolipoprotein C1 pseudogene 1 | NR_028413.1 | 29.4% | (many diffs) | |
| 6 | human | 342 | APOC1P1 | apolipoprotein C1 pseudogene 1 | NR_028412.1 | 23.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 297
- ORF length:
- 231
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag gctcttcctg tcgctcccgg tcctggtggt ggttctgtcg atcgtcttgg 121 aaggcccagc cccagcccag gggaccccag acgtctccag tgccttggat aagctgaagg 181 agtttggaaa cacactggag gacaaggctc gggaactcat cagccgcatc aaacagagtg 241 aactttctgc caagatgcgg gagtggtttt cagagacatt tcagaaagtg aaggagtaac 301 tcaagattga ctcatgccca actttcttgt acaaagtggt tggtaagcct atccctaacc 361 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 421 cgacaatcaa cctctggatt a