Construct: ORF ccsbBroad304_05870
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007901.1_s304c1, BRDN0000402710
- Derived from:
- ccsbBroadEn_05870
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP5MC1 (516)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05870
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 516 | ATP5MC1 | ATP synthase membrane subun... | NM_001002027.2 | 99.5% | 99.2% | 11C>G;303G>T |
| 2 | human | 516 | ATP5MC1 | ATP synthase membrane subun... | NM_005175.3 | 99.5% | 99.2% | 11C>G;303G>T |
| 3 | mouse | 11951 | Atp5g1 | ATP synthase, H+ transporti... | NM_001161419.1 | 89.2% | 89.7% | (many diffs) |
| 4 | mouse | 11951 | Atp5g1 | ATP synthase, H+ transporti... | NM_007506.6 | 89.2% | 89.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 474
- ORF length:
- 408
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca gaccggcggg gcattattca tttctccagc tctgatccgc tgttgtacca 121 ggggtctaat caggcctgtg tctgcctcct tcttgaatag cccagtgaat tcatctaaac 181 agccttccta cagcaacttc ccactccagg tggccagacg ggagttccag accagtgttg 241 tctcccggga cattgacaca gcagccaagt ttattggtgc tggggcagcc acagttggtg 301 tggctggttc aggggctggc attggaaccg tgtttggcag cttgatcatt ggctatgcca 361 ggaacccttc tctcaagcag cagctcttct cctatgccat tcttggcttt gccctgtctg 421 aggccatggg gcttttctgt ttgatggtcg ccttcctcat cctcttcgcc atgtgcccaa 481 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 541 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta