Construct: ORF ccsbBroad304_05913
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012196.1_s304c1, BRDN0000399214
- Derived from:
- ccsbBroadEn_05913
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C6 (729)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05913
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 729 | C6 | complement C6 | NM_000065.4 | 99.9% | 99.8% | 356C>A;1881C>T |
| 2 | human | 729 | C6 | complement C6 | NM_001115131.3 | 99.9% | 99.8% | 356C>A;1881C>T |
| 3 | human | 729 | C6 | complement C6 | XM_005248357.3 | 98.9% | 98.9% | 1_27del;383C>A;1908C>T |
| 4 | human | 729 | C6 | complement C6 | XM_011514119.3 | 98% | 98% | (many diffs) |
| 5 | human | 729 | C6 | complement C6 | XM_006714496.4 | 97% | 96.8% | (many diffs) |
| 6 | human | 729 | C6 | complement C6 | XM_011514115.3 | 97% | 96.8% | (many diffs) |
| 7 | human | 729 | C6 | complement C6 | XM_011514116.3 | 97% | 96.8% | (many diffs) |
| 8 | human | 729 | C6 | complement C6 | XM_011514117.3 | 97% | 96.8% | (many diffs) |
| 9 | human | 729 | C6 | complement C6 | XM_011514118.3 | 97% | 96.8% | (many diffs) |
| 10 | human | 729 | C6 | complement C6 | XM_017009818.2 | 97% | 96.8% | (many diffs) |
| 11 | human | 729 | C6 | complement C6 | XM_011514114.3 | 96.1% | 95.9% | (many diffs) |
| 12 | human | 729 | C6 | complement C6 | XM_017009819.2 | 81.6% | 81.6% | (many diffs) |
| 13 | human | 729 | C6 | complement C6 | XM_011514121.3 | 63.8% | 63.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2868
- ORF length:
- 2802
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc cagacgctct gtcttgtact tcatcctgct gaatgctctg atcaacaagg 121 gccaagcctg cttctgtgat cactatgcat ggactcagtg gaccagctgc tcaaaaactt 181 gcaattctgg aacccagagc agacacagac aaatagtagt agataagtac taccaggaaa 241 acttttgtga acagatttgc agcaagcagg agactagaga atgtaactgg caaagatgcc 301 ccatcaactg cctcctggga gattttggac catggtcaga ctgtgaccct tgtattgaaa 361 aacagtctaa agttagatct gtcttgcgtc ccagtcagtt tgggggacag ccatgcactg 421 agcctctggt agcctttcaa ccatgcattc catctaagct ctgcaaaatt gaagaggctg 481 actgcaagaa taaatttcgc tgtgacagtg gccgctgcat tgccagaaag ttagaatgca 541 atggagaaaa tgactgtgga gacaattcag atgaaaggga ctgtgggagg acaaaggcag 601 tatgcacacg gaagtataat cccatcccta gtgtacagtt gatgggcaat gggtttcatt 661 ttctggcagg agagcccaga ggagaagtcc ttgataactc tttcactgga ggaatatgta 721 aaactgtcaa aagcagtagg acaagtaatc cataccgtgt tccggccaat ctggaaaatg 781 tcggctttga ggtacaaact gcagaagatg acttgaaaac agatttctac aaggatttaa 841 cttctcttgg acacaatgaa aatcaacaag gctcattctc aagtcagggg gggagctctt 901 tcagtgtacc aattttttat tcctcaaaga gaagtgaaaa tatcaaccat aattctgcct 961 tcaaacaagc cattcaagcc tctcacaaaa aggattctag ttttattagg atccataaag 1021 tgatgaaagt cttaaacttc acaacgaaag ctaaagatct gcacctttct gatgtctttt 1081 tgaaagcact taaccatctg cctctagaat acaactctgc tttgtacagc cgaatattcg 1141 atgactttgg gactcattac ttcacctctg gctccctggg aggcgtgtat gaccttctct 1201 atcagtttag cagtgaggaa ctaaagaact caggtttaac cgaggaagaa gccaaacact 1261 gtgtcaggat tgaaacaaag aaacgcgttt tatttgctaa gaaaacaaaa gtggaacata 1321 ggtgcaccac caacaagctg tcagagaaac atgaaggttc atttatacag ggagcagaga 1381 aatccatatc cctgattcga ggtggaagga gtgaatatgg agcagctttg gcatgggaga 1441 aagggagctc tggtctggag gagaagacat tttctgagtg gttagaatca gtgaaggaaa 1501 atcctgctgt gattgacttt gagcttgccc ccatcgtgga cttggtaaga aacatcccct 1561 gtgcagtgac aaaacggaac aacctcagga aagctttgca agagtatgca gccaagttcg 1621 atccttgcca gtgtgctcca tgccctaata atggccgacc caccctctca gggactgaat 1681 gtctgtgtgt gtgtcagagt ggcacctatg gtgagaactg tgagaaacag tctccagatt 1741 ataaatccaa tgcagtagac ggacagtggg gttgttggtc ttcctggagt acctgtgatg 1801 ctacttataa gagatcgaga acccgagaat gcaataatcc tgccccccaa cgaggaggga 1861 aacgctgtga gggggagaag cgacaagagg aagactgcac attttcaatc atggaaaaca 1921 atggacaacc atgtatcaat gatgatgaag aaatgaaaga ggtcgatctt cctgagatag 1981 aagcagattc cgggtgtcct cagccagttc ctccagaaaa tggatttatc cggaatgaaa 2041 agcaactata cttggttgga gaagatgttg aaatttcatg ccttactggc tttgaaactg 2101 ttggatacca gtacttcaga tgcttaccag acgggacctg gagacaaggg gatgtggaat 2161 gccaacggac ggagtgcatc aagccagttg tgcaggaagt cctgacaatt acaccatttc 2221 agagattgta tagaattggt gaatccattg agctaacttg ccccaaaggc tttgttgttg 2281 ctgggccatc aaggtacaca tgccagggga attcctggac accacccatt tcaaactctc 2341 tcacctgtga aaaagatact ctaacaaaat taaaaggcca ttgtcagctg ggacagaaac 2401 aatcaggatc tgaatgcatt tgtatgtctc cagaagaaga ctgtagccat cattcagaag 2461 atctctgtgt gtttgacaca gactccaacg attactttac ttcacccgct tgtaagtttt 2521 tggctgagaa atgtttaaat aatcagcaac tccattttct acatattggt tcctgccaag 2581 acggccgcca gttagaatgg ggtcttgaaa ggacaagact ttcatccaac agcacaaaga 2641 aagaatcctg tggctatgac acctgctatg actgggaaaa atgttcagcc tccacttcca 2701 aatgtgtctg cctattgccc ccacagtgct tcaagggtgg aaaccaactc tactgtgtca 2761 aaatgggatc atcaacaagt gagaaaacat tgaacatctg tgaagtggga actataagat 2821 gtgcaaacag gaagatggaa atactgcatc ctggaaagtg tttggcctac ccaactttct 2881 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 2941 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta