Construct: ORF ccsbBroad304_05933

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007101.1_s304c1, BRDN0000411568
Derived from:
ccsbBroadEn_05933
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CAPN2 (824)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_05933
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 824 CAPN2 calpain 2 NM_001748.5 99.8% 99.8% 66C>G;306G>A;582G>A
2 human 824 CAPN2 calpain 2 NM_001146068.1 88.7% 88.7% (many diffs)
3 human 824 CAPN2 calpain 2 XR_002957688.1 40.2% (many diffs)
4 mouse 12334 Capn2 calpain 2 NM_009794.3 87.6% 94% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2166
ORF length:
2100
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc gggcatcgcg gccaagctgg cgaaggaccg ggaggcggcc gaggggctgg
121gctcccacga gagggccatc aagtacctca accaggacta cgaggcgctg cggaacgagt
181gcctggaggc cgggacgctc ttccaggacc cgtccttccc ggccatcccc tcggccctgg
241gcttcaagga gttggggccc tactccagca aaacccgggg catcgagtgg aagcgcccca
301cggagatctg cgctgacccc cagtttatca ttggaggagc cacccgcaca gacatctgcc
361aaggagccct aggtgactgc tggctgctgg cagccattgc ctccctcacc ttgaatgaag
421aaatcctggc tcgagtcgtc cccctaaacc agagcttcca ggaaaactat gcagggatct
481ttcacttcca gttctggcaa tacggcgagt gggtggaggt ggtggtggat gacaggctgc
541ccaccaagga cggggagctg ctctttgtgc attcagccga agggagcgag ttctggagcg
601ccctgctgga gaaggcatac gccaagatca acggatgcta tgaagcacta tcagggggtg
661ccaccactga gggcttcgaa gacttcaccg gaggcattgc tgagtggtat gagttgaaga
721agccccctcc caacctgttc aagatcatcc agaaagctct gcaaaaaggc tctctccttg
781gctgctccat cgacatcacc agcgccgcgg actcggaggc catcacgttt cagaagctgg
841tgaaggggca cgcgtactcg gtcaccggag ccgaggaggt tgaaagtaac ggaagcctac
901agaaactgat ccgcatccga aatccctggg gagaagtgga gtggacaggg cggtggaatg
961acaactgccc aagctggaac actatagacc cagaggagag ggaaaggctg accagacggc
1021atgaagatgg agaattctgg atgtctttca gtgacttcct gaggcactat tcccgcctgg
1081agatctgtaa cctgacccca gacactctca ccagcgatac ctacaagaag tggaaactca
1141ccaaaatgga tgggaactgg aggcggggct ccaccgcggg aggttgcagg aactacccga
1201acacattctg gatgaaccct cagtacctga tcaagctgga ggaggaggat gaggacgagg
1261aggatgggga gagcggctgc accttcctgg tggggctcat tcagaagcac cgacggcggc
1321agaggaagat gggcgaggac atgcacacca tcggctttgg catctatgag gttccagagg
1381agttaagtgg gcagaccaac atccacctca gcaaaaactt cttcctgacg aatcgcgcca
1441gggagcgctc agacaccttc atcaacctcc gggaggtgct caaccgcttc aagctgccgc
1501caggagagta cattctcgtg ccttccacct tcgaacccaa caaggatggg gatttctgca
1561tccgggtctt ttctgaaaag aaagctgact accaagctgt cgatgatgaa atcgaggcca
1621atcttgaaga gttcgacatc agcgaggatg acattgatga tggattcagg agactgtttg
1681cccagttggc aggagaggat gcggagatct ctgcctttga gctgcagacc atcctgagaa
1741gggttctagc aaagcgccaa gatatcaagt cagatggctt cagcatcgag acatgcaaaa
1801ttatggttga catgctagat tcggacggga gtggcaagct ggggctgaag gagttctaca
1861ttctctggac gaagattcaa aaataccaaa aaatttaccg agaaatcgac gttgacaggt
1921ctggtaccat gaattcctat gaaatgcgga aggcattaga agaagcaggt ttcaagatgc
1981cctgtcaact ccaccaagtc atcgttgctc ggtttgcaga tgaccagctc atcatcgatt
2041ttgataattt tgttcggtgt ttggttcggc tggaaacgct attcaagata tttaagcagc
2101tggatcccga gaatactgga acaatagagc tcgaccttat ctcttggctc tgtttctcag
2161tactttgccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
2221gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
2281acctctggat ta

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