Construct: ORF ccsbBroad304_06001
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015601.1_s304c1, BRDN0000408696
- Derived from:
- ccsbBroadEn_06001
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHRNA4 (1137)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06001
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1137 | CHRNA4 | cholinergic receptor nicoti... | NM_000744.6 | 99.5% | 99.8% | (many diffs) |
| 2 | human | 1137 | CHRNA4 | cholinergic receptor nicoti... | XM_011528524.1 | 78.8% | 77.6% | (many diffs) |
| 3 | human | 1137 | CHRNA4 | cholinergic receptor nicoti... | NM_001256573.2 | 71.6% | 71.9% | (many diffs) |
| 4 | human | 1137 | CHRNA4 | cholinergic receptor nicoti... | XM_017027625.2 | 71.6% | 71.9% | (many diffs) |
| 5 | human | 1137 | CHRNA4 | cholinergic receptor nicoti... | XM_024451822.1 | 71.6% | 71.9% | (many diffs) |
| 6 | human | 1137 | CHRNA4 | cholinergic receptor nicoti... | NR_046317.2 | 33.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1950
- ORF length:
- 1881
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagctagag ggccccggag cgccgcggct gctgccgccg ctgctgctac 121 ttctggggac cggcctcctg cgcgccagca gccatgtgga gacccgggcc cacgccgagg 181 agcggctcct gaagaaactc ttctccggtt acaacaagtg gtcccgaccc gtggccaaca 241 tctcggacgt ggtcctcgtc cgcttcggcc tgtccatcgc tcagctcatt gacgtggatg 301 agaagaacca gatgatgacc acgaacgtat gggtgaagca ggagtggcac gactacaagc 361 tgcgctggga cccagctgac tatgagaatg tcacctccat ccgcatcccc tccgagctca 421 tctggcggcc ggacatcgtc ctctacaaca atgctgacgg ggacttcgcg gtcacccacc 481 tgaccaaggc ccacctgttc catgacgggc gggtgcagtg gactcccccg gccatttaca 541 agagctcctg cagcatcgac gtcaccttct tccccttcga ccagcagaac tgcaccatga 601 aattcggctc ctggacctac gacaaggcca agatcgacct ggtgaacatg cacagccgcg 661 tggaccagct ggacttctgg gagagtggcg agtgggtcat cgtggacgcc gtgggcacct 721 acaacaccag gaagtacgag tgctgcgccg agatctaccc ggacatcacc tatgccttcg 781 tcatccggcg gctgccgctc ttctacacca tcaacctcat catcccctgc ctgctcatct 841 cctgcctcac cgtgctggtc ttctacctgc cctccgagtg tggcgagaag atcacgctgt 901 gcatctccgt gctgctgtcg ctcaccgtct tcctgctgct catcaccgag atcatcccgt 961 ccacctcact ggtcatccca ctcatcggcg agtacctgct gttcaccatg atcttcgtca 1021 ccctgtccat cgtcatcacg gtcttcgtgc tcaacgtgca ccaccgctcg ccacgcacgc 1081 acaccatgcc cacctgggta cgcagggtct tcctggacat cgtgccacgc ctgctcctca 1141 tgaagcggcc gtccgtggtc aaggacaatt gccggcggct catcgagtcc atgcataaga 1201 tggccagtgc cccgcgcttc tggcccgagc cagaagggga gccccctgcc acgagcggca 1261 cccagagcct gcaccctccc tcaccgtcct tctgcgtccc cctggatgtg ccggctgagc 1321 ctgggccttc ctgcaagtca ccctccgacc agctccctcc tcagcagccc ctggaagctg 1381 agaaagccag cccccacccc tcgcctggac cctgccgccc gccccacggc acccaggcac 1441 cagggctggc caaagccagg tccctcagcg tccagcacat gtccagccct ggcgaagcgg 1501 tggaaggcgg cgtccggtgc cggtctcgga gcatccagta ctgtgttccc cgagacgatg 1561 ccgcccccga ggcagatggc caggctgccg gcgccctggc ctctcgcaac acccactcgg 1621 ctgagctccc acccccagac cagccctctc cgtgcaaatg cacatgcaag aaggagccct 1681 cttcggtgtc cccgagtgcc acggtcaaga cccgcagcac caaagcaccg cccccgcacc 1741 tgcccctgtc gccggccctg acccgggcgg tggagggcgt ccagtacatt gcagaccacc 1801 tgaaggccga agacacagac ttctcggtga aggaggactg gaagtacgtg gccatggtca 1861 tcgaccgcat cttcctctgg atgttcatca tcgtctgcct gctggggacg gtgggcctct 1921 tcctgccgcc ctggctggct ggcatgatct tgccaacttt cttgtacaaa gtggttggta 1981 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 2041 ggtggcgcgt taagtcgaca atcaacctct ggatta