Construct: ORF ccsbBroad304_06009
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016700.1_s304c1, BRDN0000401771
- Derived from:
- ccsbBroadEn_06009
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLC (1178)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06009
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1178 | CLC | Charcot-Leyden crystal gale... | NM_001828.6 | 99.5% | 99.2% | 83C>T;240T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 495
- ORF length:
- 426
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtccctgcta cccgtgccat acacagaggc tgcctctttg tctactggtt 121 ctactgtgac aatcaaaggg cgaccacttg tctgtttctt gaatgaacca tatctgcagg 181 tggatttcca cactgagatg aaggaggaat cagacattgt cttccatttc caagtgtgct 241 ttggtcgtcg tgtggtcatg aacagccgtg agtatggggc ctggaagcag caggtggaat 301 ccaagaacat gccctttcag gatggccaag aatttgaact gagcatctca gtgctgccag 361 ataagtacca ggtaatggtc aatggccaat cctcttacac ctttgaccat agaatcaagc 421 ctgaggctgt gaagatggtg caagtgtgga gagatatctc cctgaccaaa tttaatgtca 481 gctatttaaa gagattgcca actttcttgt acaaagtggt tggtaagcct atccctaacc 541 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 601 cgacaatcaa cctctggatt a