Construct: ORF ccsbBroad304_06023
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012630.1_s304c1, BRDN0000399038
- Derived from:
- ccsbBroadEn_06023
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COX10 (1352)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06023
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1352 | COX10 | cytochrome c oxidase assemb... | NM_001303.4 | 99.7% | 99.7% | 476G>A;504G>A;699A>G |
| 2 | mouse | 70383 | Cox10 | cytochrome c oxidase assemb... | XM_006534168.3 | 43.5% | 45.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1395
- ORF length:
- 1329
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc cgcatctccg cacactctct cctcacgcct cctgacaggt tgcgtaggag 121 gctctgtctg gtatcttgaa agaagaacta tacaggactc ccctcacaag ttcttacatc 181 ttctcaggaa tgtcaataag cagtggatta catttcagca ctttagcttc ctcaaacgca 241 tgtatgtcac acagctgaac agaagccaca accagcaagt aagacccaag ccagaaccag 301 tagcatctcc tttccttgaa aaaacatctt caggtcaagc caaagcagaa atatatgaga 361 tgagacctct ctcaccgccc agcctatctt tgtccagaaa gccaaatgaa aaggaattga 421 tagaactaga gccagactca gtaattgaag actcaataga tgtagggaaa gagacaaaag 481 aggaaaagcg gtggaaagag atgaagctgc aagtgtatga tttgccagga attttggctc 541 aactatccaa aatcaaactc acagctctag ttgtaagtac cactgcagct ggatttgcat 601 tggctccggg cccttttgac tggccctgtt tcctgcttac ttctgttggg acaggccttg 661 catcctgtgc tgccaactcc atcaatcagt tttttgaggt gccatttgac tcaaacatga 721 ataggacaaa gaacagaccg ctggttcgtg gacagatcag cccgttgcta gctgtgtcct 781 ttgccacttg ttgtgctgtt ccgggagttg ccattctgac cttgggggtg aatccactca 841 caggagccct ggggctcttc aacattttcc tgtatacctg ctgctacaca ccactgaaaa 901 ggatcagcat tgccaacaca tgggtcggag ctgtggttgg ggccatcccg cctgtcatgg 961 gctggacagc ggccacgggc agcctcgatg ctggcgcatt tctcctggga ggaatcctct 1021 actcctggca gtttcctcat ttcaacgccc tgagctgggg cctccgtgaa gactactccc 1081 ggggcggcta ctgcatgatg tcggtcaccc acccgggcct gtgccggcgc gtggcgctgc 1141 gccactgcct ggccctgctc gtgctgtccg cagcagcccc tgtgctggac atcaccacat 1201 ggaccttccc catcatggcc cttcccatca atgcgtacat ctcctacctc ggcttccgct 1261 tctacgtgga cgcagaccgc aggagctcgc ggagactgtt cttctgcagc ctgtggcacc 1321 tgccgctgct gctgctgctc atgctcacct gcaagcggcc gagcggaggc ggggacgcag 1381 ggccccctcc cagctgccca actttcttgt acaaagtggt tggtaagcct atccctaacc 1441 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 1501 cgacaatcaa cctctggatt a