Construct: ORF ccsbBroad304_06032
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005069.1_s304c1, BRDN0000399083
- Derived from:
- ccsbBroadEn_06032
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CPN1 (1369)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06032
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1369 | CPN1 | carboxypeptidase N subunit 1 | NM_001308.3 | 99.9% | 100% | 846G>A |
| 2 | mouse | 93721 | Cpn1 | carboxypeptidase N, polypep... | NM_030703.2 | 84.4% | 83.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1440
- ORF length:
- 1374
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc agacctgctc tcagtcttcc tccacctcct ccttctcttc aagttggttg 121 ccccggtgac ctttcgccac caccgctatg atgatcttgt gcggacgctg tacaaggtgc 181 aaaacgaatg ccccggcatc acgcgggtct acagcattgg gcgcagcgtg gaggggagac 241 acctctacgt gctggagttc agcgaccacc ctggaatcca cgagcccttg gaaccagagg 301 tcaagtatgt ggggaacatg cacggcaacg aagcgttggg ccgcgagctg atgctgcagc 361 tgtcggagtt tctgtgcgag gagttccgga acaggaacca gcgcatcgtc cagctcatcc 421 aggacacgcg cattcacatc ctgccatcca tgaaccccga cggctacgag gtggctgctg 481 cccagggccc aaacaagcct gggtatctag ttggcaggaa caatgcaaat ggagtggacc 541 tgaaccgcaa cttccctgat ctcaatacct atatctacta taacgagaag tacggaggcc 601 ccaaccacca cctgcccctt ccagacaact ggaaaagtca ggtggaaccc gagacccggg 661 cggtgatccg gtggatgcac tccttcaact ttgttctttc agccaatctc cacggagggg 721 cggtggtggc caattacccg tatgacaagt cctttgagca ccgggtccga ggggtccgcc 781 gcaccgccag cacccccacg cctgacgaca agctcttcca gaagctggcc aaggtctact 841 cctatgcaca tggatggatg ttccaaggtt ggaactgcgg agattacttc ccagatggca 901 tcaccaatgg agcttcctgg tattctctca gcaagggaat gcaagacttt aattatctcc 961 ataccaactg ctttgagatc acgctggaac tgagttgcga caagtttccc cccgaagagg 1021 agttacagcg ggagtggctg ggtaatcggg aagccctaat ccagttcctg gaacaggttc 1081 accagggcat caagggaatg gtgcttgatg agaattacaa taatctcgcc aatgctgtca 1141 tttctgtcag tgggattaac catgatgtca cttcaggtga ccatggtgat tacttccggc 1201 tgctgcttcc aggtatctac actgttagtg ccacagcacc tgggtatgac ccagagacag 1261 taactgtgac cgtgggtcct gcggaaccaa cgttggttaa cttccacctc aaaagaagca 1321 tccctcaagt aagccctgtg aggagagctc ccagcagaag gcacggagtc agagccaaag 1381 tgcagcccca agccagaaag aaagaaatgg agatgaggca gctgcagaga ggccctgcct 1441 gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 1501 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 1561 ggatta