Construct: ORF ccsbBroad304_06046

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015391.1_s304c1, BRDN0000408209
Derived from:
ccsbBroadEn_06046
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CRYGD (1421)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06046
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1421 CRYGD crystallin gamma D NM_006891.4 99.6% 100% 51T>C;285A>G
2 mouse 12967 Crygd crystallin, gamma D NM_007776.2 88.5% 84.4% (many diffs)
3 mouse 12968 Cryge crystallin, gamma E NM_007777.3 86.8% 79.8% (many diffs)
4 mouse 12969 Crygf crystallin, gamma F NM_027010.2 86.5% 79.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
591
ORF length:
522
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggggaagatc accctctacg aggaccgggg cttccagggc cgccactacg
121aatgcagcag cgaccacccc aacctgcagc cctacttgag ccgctgcaac tcggcgcgcg
181tggacagcgg ctgctggatg ctctatgagc agcccaacta ctcgggcctc cagtacttcc
241tgcgccgcgg cgactatgcc gaccaccagc agtggatggg cctcagcgac tcggtccgct
301cctgccgcct catcccccac tctggctctc acaggatcag actctatgag agggaggact
361acagaggcca gatgatagag ttcactgagg actgctcctg tcttcaggac cgcttccgct
421tcaatgaaat ccactccctc aacgtgctgg agggctcctg ggtcctctac gagctgtcca
481actaccgagg acggcagtac ctgctgatgc caggggacta taggcgctac caggactggg
541gggccacgaa tgccagagtg ggctctctga ggagagtcat agatttctcc ttgccaactt
601tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt
661agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)