Construct: ORF ccsbBroad304_06077
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018104.1_s304c1, BRDN0000400212
- Derived from:
- ccsbBroadEn_06077
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP11A1 (1583)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06077
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1583 | CYP11A1 | cytochrome P450 family 11 s... | NM_000781.3 | 99.9% | 99.8% | 8C>T |
| 2 | human | 1583 | CYP11A1 | cytochrome P450 family 11 s... | NM_001099773.2 | 69.6% | 69.6% | 0_1ins474 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1629
- ORF length:
- 1563
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct ggtcaagggt cttcccccac gctcagtcct ggtcaaaggc tgccagacct 121 ttctgagtgc ccccagggag gggctggggc gtctcagggt gcccactggc gagggagctg 181 gcatctccac ccgcagtcct cgccccttca atgagatccc ctctcctggt gacaatggct 241 ggctaaacct gtaccatttc tggagggaga cgggcacaca caaagtccac cttcaccatg 301 tccagaattt ccagaagtat ggcccgattt acagggagaa gctcggcaac gtggagtcgg 361 tttatgtcat cgaccctgaa gatgtggccc ttctctttaa gtccgagggc cccaacccag 421 aacgattcct catcccgccc tgggtcgcct atcaccagta ttaccagaga cccataggag 481 tcctgttgaa gaagtcggca gcctggaaga aagaccgggt ggccctgaac caggaggtga 541 tggctccaga ggccaccaag aactttttgc ccctgttgga tgcagtgtct cgggacttcg 601 tcagtgtcct gcacaggcgc atcaagaagg cgggctccgg aaattactcg ggggacatca 661 gtgatgacct gttccgcttt gcctttgagt ccatcactaa cgtcattttt ggggagcgcc 721 aggggatgct ggaggaagta gtgaaccccg aggcccagcg attcattgat gccatctacc 781 agatgttcca caccagcgtc cccatgctca accttccccc agacctgttc cgtctgttca 841 ggaccaagac ctggaaggac catgtggctg catgggacgt gattttcagt aaagctgaca 901 tatacaccca gaacttctac tgggaattga gacagaaagg aagtgttcac cacgattacc 961 gtggcatcct ctacagactc ctgggagaca gcaagatgtc cttcgaggac atcaaggcca 1021 acgtcacaga gatgctggca ggaggggtgg acacgacgtc catgaccctg cagtggcact 1081 tgtatgagat ggcacgcaac ctgaaggtgc aggatatgct gcgggcagag gtcttggctg 1141 cgcggcacca ggcccaggga gacatggcca cgatgctaca gctggtcccc ctcctcaaag 1201 ccagcatcaa ggagacacta agacttcacc ccatctccgt gaccctgcag agatatcttg 1261 taaatgactt ggttcttcga gattacatga ttcctgccaa gacactggtg caagtggcca 1321 tctatgctct gggccgagag cccaccttct tcttcgaccc ggaaaatttt gacccaaccc 1381 gatggctgag caaagacaag aacatcacct acttccggaa cttgggcttt ggctggggtg 1441 tgcggcagtg tctgggacgg cggatcgctg agctagagat gaccatcttc ctcatcaata 1501 tgctggagaa cttcagagtt gaaatccaac acctcagcga tgtgggcacc acattcaacc 1561 tcattctgat gcctgaaaag cccatctcct tcaccttctg gccctttaac caggaagcaa 1621 cccagcagtg cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 1681 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 1741 tcaacctctg gatta