Construct: ORF ccsbBroad304_06082

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015214.1_s304c1, BRDN0000408780
Derived from:
ccsbBroadEn_06082
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DGKG (1608)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06082
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1608 DGKG diacylglycerol kinase gamma NM_001080745.2 99.8% 99.7% (many diffs)
2 human 1608 DGKG diacylglycerol kinase gamma NM_001346.3 94.9% 94.8% (many diffs)
3 human 1608 DGKG diacylglycerol kinase gamma NM_001080744.2 91.7% 91.6% (many diffs)
4 mouse 110197 Dgkg diacylglycerol kinase, gamma NM_001346673.1 87.1% 89.2% (many diffs)
5 mouse 110197 Dgkg diacylglycerol kinase, gamma NM_138650.2 82.8% 84.8% (many diffs)
6 mouse 110197 Dgkg diacylglycerol kinase, gamma XM_017316851.1 82% 84% (many diffs)
7 mouse 110197 Dgkg diacylglycerol kinase, gamma XM_017316850.1 78.2% 80.1% (many diffs)
8 mouse 110197 Dgkg diacylglycerol kinase, gamma XM_017316852.1 68.6% 69.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2325
ORF length:
2256
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gggtgaagaa cggtgggtct ccctcactcc agaagaattt gaccaactcc
121agaaatattc agaatattcc tccaagaaga taaaagatgc cttgactgaa tttaatgagg
181gtgggagcct caaacaatat gacccacatg agccgattag ctatgatgtc ttcaagctgt
241tcatgagggc gtacctagag gtggaccttc cccagccact gagcactcac ctcttcctgg
301ccttcagcca gaagcccaga cacgagacct ctgaccaccc gacggaggga gccagcaaca
361gtgaggccaa cagcgcagat actaatatac agaatgcaga taatgccacc aaagcagacg
421aggcctgtgc ccctgatact gaatcaaata tggctgagaa gcaagcacca gctgaagacc
481aagtggctgc gagccccctg gaaccccccg tccctcggtc ttcaagctcg gaatccccag
541tggtgtacct gaaggatgtt gtgtgctacc tgtccctgct ggagacgggg aggcctcagg
601ataagctgga gttcatgttt cgcctctatg attcagatga gaacggtctc ctggaccaag
661cggagatgga ttgcattgtc aaccaaatgc tgcatattgc ccagtacctg gagtgggatc
721ccacagagct gaggcctata ttgaaggaga tgctgcaagg gatggactac gaccgggacg
781gctttgtgtc tctacaggaa tgggtccatg gagggatgac caccatccca ttgctggtcc
841tcctggggat ggatgactct ggctccaagg gggatgggcg gcacgcctgg accatgaagc
901acttcaagaa accaacctac tgcaacttct gccatatcat gctcatgggc gtccgcaagc
961aaggcctgtg ctgcacttac tgtaaataca ctgtccacga acgctgtgtg tccaaaaaca
1021ttcctggttg tgtcaaaacg tactcaaaag ccaaaaggag tggtgagttt caccgcaaat
1081gtgaattatc aacgttgtgt gacggtgggg aactcagaga ccacatctta ctgcccacct
1141ccatatgccc catcacccgg gacaggccag gtgagaagtc tgatggctgc gtgtccgcca
1201agggcgaact tgtcatgcag tataagatca tccccacccc gggtacccac cccctgctgg
1261tcttggtgaa ccccaagagt ggagggagac aaggagaaag aattcttcgg aaattccact
1321atctgctcaa ccccaaacaa gttttcaacc tggacaatgg ggggcctact ccagggttga
1381actttttccg tgatactcca gacttccgtg ttttggcctg tggtggagat gggacagttg
1441gctggatttt ggattgcatt gataaggcca actttgcaaa gcatccacca gtggctgtcc
1501tgcctcttgg aacaggaaat gaccttgccc gttgtctccg ctggggagga ggttatgaag
1561ggggcagctt gacaaaaatc ctgaaagaca ttgagcagag ccccttggtg atgctggacc
1621gctggcatct ggaagtcatc cccagagagg aagtggaaaa cggggaccag gtcccataca
1681gcatcatgaa caactatttc tccattggtg tggacgcttc cattgcacac agattccatg
1741tgatgagaga gaaacatcct gaaaaattca acagcaggat gaagaacaag ctgtggtact
1801ttgaatttgg cacctcggag acttttgcag cgacctgcaa gaaactccac gaccacattg
1861agttggagtg tgatggggtt ggggtggacc tgagcaacat cttcctggaa ggcattgcca
1921ttctcaacat tcccagcatg tacggaggca ccaatctctg gggagaaaac aagaagaacc
1981gggctgtgat ccgggaaagc aggaagggtg tcactgaccc caaagaactg aaattctgcg
2041ttcaagacct cagtgaccag ctccttgaag tggtggggct agaaggagcc atggagatgg
2101ggcagatcta caccggcctg aagagtgcag gcaggaggct ggcccagtgc gcctctgtca
2161ccatcaggac aaacaagctg ctgccaatgc aagtggatgg agaaccctgg atgcagccat
2221gttgcacgat taaaattact cacaagaacc aagcgcccat gatgatgggg cctccccaga
2281agagcagctt cttctcgttg agaaggaaga gccgttcaaa agacttgcca actttcttgt
2341acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
2401gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

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