Construct: ORF ccsbBroad304_06099
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017058.1_s304c1, BRDN0000461227
- Derived from:
- ccsbBroadEn_06099
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DHODH (1723)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06099
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1723 | DHODH | dihydroorotate dehydrogenas... | NM_001361.5 | 99.9% | 99.7% | 926G>T |
| 2 | human | 1723 | DHODH | dihydroorotate dehydrogenas... | XM_005255827.4 | 92.8% | 92.6% | 0_1ins84;842G>T |
| 3 | human | 1723 | DHODH | dihydroorotate dehydrogenas... | XM_017022990.2 | 72.3% | 72.1% | 0_1ins327;599G>T |
| 4 | human | 1723 | DHODH | dihydroorotate dehydrogenas... | XM_005255829.4 | 63.6% | 63% | 1_1delAins422;4_5insCATTAACA;497G>T |
| 5 | mouse | 56749 | Dhodh | dihydroorotate dehydrogenase | NM_020046.3 | 84.9% | 87.3% | (many diffs) |
| 6 | mouse | 56749 | Dhodh | dihydroorotate dehydrogenase | XR_387762.3 | 37.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1254
- ORF length:
- 1185
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgtggaga cacctgaaaa agcgggccca ggatgctgtg atcatcctgg 121 ggggaggagg acttctcttc gcctcctacc tgatggccac gggagatgag cgtttctatg 181 ctgaacacct gatgccgact ctgcaggggc tgctggaccc ggagtcagcc cacagactgg 241 ctgttcgctt cacctccctg gggctccttc cacgggccag atttcaagac tctgacatgc 301 tggaagtgag agttctgggc cataaattcc gaaatccagt aggaattgct gcaggatttg 361 acaagcatgg ggaagccgtg gacggacttt ataagatggg ctttggtttt gttgagatag 421 gaagtgtgac tccaaaacct caggaaggaa accctagacc cagagtcttc cgcctccctg 481 aggaccaagc tgtcattaac aggtatggat ttaacagtca cgggctttca gtggtggaac 541 acaggttacg ggccagacag cagaagcagg ccaagctcac agaagatgga ctgcctctgg 601 gggtcaactt ggggaagaac aagacctcag tggacgccgc ggaggactac gcagaagggg 661 tgcgcgtact gggccccctg gccgactacc tggtggtgaa tgtgtccagc cccaacactg 721 ccgggctgcg gagccttcag ggaaaggccg agctgcgccg cctgctgacc aaggtgctgc 781 aggagaggga tggcttgcgg agagtgcaca ggccggcagt cctggtgaag atcgctcctg 841 acctcaccag ccaggataag gaggacattg ccagtgtggt caaagagttg ggcatcgatg 901 ggctgattgt tacgaacacc accgtgagtc gccctgcggg cctccagggt gccctgcgct 961 ctgaaacagg agggctgagt gggaagcccc tcctggattt atcaactcaa accattcggg 1021 agatgtatgc actcacccaa ggccgagttc ccataattgg ggttggtggt gtgagcagcg 1081 ggcaggacgc gctggagaag atccgggcag gggcctccct ggtgcagctg tacacggccc 1141 tcaccttctg ggggccaccc gttgtgggca aagtcaagcg ggaactggag gcccttctga 1201 aagagcaggg ctttggcgga gtcacagatg ccattggagc agatcatcgg aggttgccaa 1261 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 1321 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta