Construct: ORF ccsbBroad304_06119

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004186.1_s304c1, BRDN0000408056
Derived from:
ccsbBroadEn_06119
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DPYSL3 (1809)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06119
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1809 DPYSL3 dihydropyrimidinase like 3 NM_001197294.2 99.9% 99.8% 982G>A
2 human 1809 DPYSL3 dihydropyrimidinase like 3 NM_001387.3 82.6% 81.8% (many diffs)
3 human 1809 DPYSL3 dihydropyrimidinase like 3 XM_011537574.2 81.9% 80.9% (many diffs)
4 mouse 22240 Dpysl3 dihydropyrimidinase-like 3 NM_001291455.1 89.7% 96.9% (many diffs)
5 mouse 22240 Dpysl3 dihydropyrimidinase-like 3 NM_009468.5 75% 80.5% (many diffs)
6 mouse 22240 Dpysl3 dihydropyrimidinase-like 3 NM_001136086.2 74.1% 80.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2118
ORF length:
2052
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ctcgggccgg aggggctggg acagctccca cgaagacgat ctgcccgtgt
121acctggccag gccgggcacc acggaccagg tcccgcggca gaaatacggc ggcatgttct
181gcaacgtgga gggcgccttc gagagcaaga cgctggattt cgatgccctc agcgtggggc
241agcggggcgc gaagactcct cggagcggcc agggcagcga ccgaggatcg gggagtcggc
301ccgggatcga gggggacacc ccgcgcaggg gccaaggccg ggaagagagc agggagcccg
361cgcccgcctc ccccgccccc gccggggtag agatccggag cgccaccggc aaagaggtgt
421tgcagaacct cggccccaag gacaagagtg accgtctcct tatcaaggga ggcagaatcg
481tcaatgatga tcagtccttt tatgctgata tttacatgga agatggctta ataaaacaaa
541ttggagacaa tctgattgtt cctggaggag tgaagaccat tgaagccaat gggaagatgg
601tgatccctgg aggcatcgat gtccatactc acttccagat gccatataag ggaatgacca
661cagtagatga cttcttccaa gggacaaagg cggccttagc aggtggcacc accatgatca
721ttgaccatgt ggtgcctgag cctgagtcca gcctgactga ggcctatgag aaatggagag
781agtgggctga tgggaagagt tgctgtgact atgccctgca tgtggacatc acccactgga
841atgacagcgt caagcaggaa gtgcagaacc tcatcaagga caaaggggtt aactccttca
901tggtttatat ggcttataag gatttgtatc aagtatctaa cacagagctc tatgagatct
961tcacctgcct gggagagctg ggggccattg ctcaagttca tgctgagaat ggggatatca
1021ttgcccagga gcaaacccgc atgttgaaaa tggggataac tggcccagaa ggccatgtac
1081tgagcaggcc agaagagctg gaagctgagg ctgtgttccg tgccatcacc attgccagcc
1141aaaccaattg ccctctctac gtcacaaagg tcatgagcaa gagtgcagct gacctcatct
1201cacaagccag gaaaaaagga aatgtagtct ttggtgagcc catcactgcc agcctcggca
1261tagatggaac ccattattgg agcaagaact gggccaaggc ggctgcattt gtgacatccc
1321cacccctgag ccctgaccca actactccgg actacatcaa ctccttgctg gccagcgggg
1381atctgcagct atctgggagt gcccactgca ccttcagcac tgcccagaaa gcaattggga
1441aggacaactt cacagccatt cctgagggca ccaatggtgt ggaggagcgg atgtctgtca
1501tctgggacaa ggctgtggcc acagggaaaa tggacgaaaa ccagttcgtg gctgtgacaa
1561gcacaaacgc tgccaagatc ttcaacctgt atccccgcaa gggaagaata tctgtgggtt
1621ctgacagcga cctcgtcatc tgggatccag atgctgtgaa gatcgtctct gccaagaacc
1681accagtctgc ggcagagtac aacatctttg aagggatgga gctgcgcggg gctcctctgg
1741ttgtcatctg ccagggcaag atcatgctgg aagatggcaa cctgcacgtg acccaggggg
1801ctggccgctt cataccctgc agcccgttct ccgactatgt ctacaagcgc attaaagcac
1861ggaggaagat ggcagacctg catgccgtcc caaggggcat gtacgatggg cctgtgtttg
1921acctgaccac cacccccaaa ggtggcaccc ccgcaggctc tgctcggggc tctcctactc
1981ggccgaaccc acctgtgagg aatcttcatc agtcgggatt tagcctgtca ggcacccaag
2041tggatgaggg ggttcgctca gccagcaagc gcatcgtggc gcccccaggc ggccgttcta
2101atatcacatc tctgagttac ccaactttct tgtacaaagt ggttggtaag cctatcccta
2161accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
2221agtcgacaat caacctctgg atta

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