Construct: ORF ccsbBroad304_06130

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007082.1_s304c1, BRDN0000399965
Derived from:
ccsbBroadEn_06130
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DUSP4 (1846)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06130
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1846 DUSP4 dual specificity phosphatase 4 NM_001394.7 99.9% 100% 522T>C
2 human 1846 DUSP4 dual specificity phosphatase 4 NM_057158.3 68.4% 65.1% (many diffs)
3 human 1846 DUSP4 dual specificity phosphatase 4 XM_011544428.3 68.4% 65.1% (many diffs)
4 mouse 319520 Dusp4 dual specificity phosphatase 4 NM_176933.4 89% 95.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1248
ORF length:
1182
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggt gacgatggag gagctgcggg agatggactg cagtgtgctc aaaaggctga
121tgaaccggga cgagaatggc ggcggcgcgg gcggcagcgg cagccacggc accctggggc
181tgccgagcgg cggcaagtgc ctgctgctgg actgcagacc gttcctggcg cacagcgcgg
241gctacatcct aggttcggtc aacgtgcgct gtaacaccat cgtgcggcgg cgggctaagg
301gctccgtgag cctggagcag atcctgcccg ccgaggagga ggtacgcgcc cgcttgcgct
361ccggcctcta ctcggcggtc atcgtctacg acgagcgcag cccgcgcgcc gagagcctcc
421gcgaggacag caccgtgtcg ctggtggtgc aggcgctgcg ccgcaacgcc gagcgcaccg
481acatctgcct gctcaaaggc ggctatgaga ggttttcctc cgagtaccca gaattctgtt
541ctaaaaccaa ggccctggca gccatcccac ccccggttcc ccccagcgcc acagagccct
601tggacctggg ctgcagctcc tgtgggaccc cactacacga ccaggggggt cctgtggaga
661tccttccctt cctctacctc ggcagtgcct accatgctgc ccggagagac atgctggacg
721ccctgggcat cacggctctg ttgaatgtct cctcggactg cccaaaccac tttgaaggac
781actatcagta caagtgcatc ccagtggaag ataaccacaa ggccgacatc agctcctggt
841tcatggaagc catagagtac atcgatgccg tgaaggactg ccgtgggcgc gtgctggtgc
901actgccaggc gggcatctcg cggtcggcca ccatctgcct ggcctacctg atgatgaaga
961aacgggtgag gctggaggag gccttcgagt tcgttaagca gcgccgcagc atcatctcgc
1021ccaacttcag cttcatgggg cagctgctgc agttcgagtc ccaggtgctg gccacgtcct
1081gtgctgcgga ggctgctagc ccctcgggac ccctgcggga gcggggcaag acccccgcca
1141cccccacctc gcagttcgtc ttcagctttc cggtctccgt gggcgtgcac tcggccccca
1201gcagcctgcc ctacctgcac agccccatca ccacctctcc cagctgttac ccaactttct
1261tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
1321aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

Download FASTA (ORF) (Full)