Construct: ORF ccsbBroad304_06151

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004572.1_s304c1, BRDN0000399003
Derived from:
ccsbBroadEn_06151
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EIF5 (1983)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06151
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1983 EIF5 eukaryotic translation init... NM_001969.5 99.9% 99.7% 18C>G
2 human 1983 EIF5 eukaryotic translation init... NM_183004.5 99.9% 99.7% 18C>G
3 mouse 217869 Eif5 eukaryotic translation init... NM_173363.5 92.3% 96.9% (many diffs)
4 mouse 217869 Eif5 eukaryotic translation init... NM_178041.2 92.3% 96.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1359
ORF length:
1293
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtc tgtcaatgtc aagcgcagcg tgtcagacca gttctatcgc tacaagatgc
121cccgtctgat tgccaaggtt gagggcaaag gcaatggaat caagacagtt atagtcaaca
181tggttgacgt tgcaaaggcg cttaatcggc ctccaacgta tcccaccaaa tattttggtt
241gtgagctggg agcacagacc cagtttgatg ttaagaatga ccgttacatt gtcaatggat
301ctcatgaggc gaataagctg caagacatgt tggatggatt cattaaaaaa tttgttctct
361gtcctgaatg tgagaatcct gaaacagatt tgcatgtcaa tccaaagaag caaacaatag
421gtaattcttg taaagcctgt ggctatcgag gcatgcttga cacacatcat aaactctgca
481cattcattct caaaaaccca cctgagaata gtgacagtgg tacaggaaag aaagaaaaag
541aaaagaaaaa cagaaagggc aaagacaagg aaaatggctc cgtatccagc agtgagacac
601caccaccacc accaccacca aatgaaatta atcctcctcc acatacaatg gaagaagagg
661aggatgatga ctggggagaa gatacaactg aggaagctca aaggcgtcga atggatgaaa
721tcagtgacca tgcaaaagtt ctgacactca gtgatgattt ggaaagaaca attgaggaga
781gggtcaatat cctctttgat tttgttaaga aaaagaaaga agagggtgtt attgattcat
841ctgacaaaga aatcgttgct gaagcagaaa gactggatgt aaaagccatg ggccctcttg
901ttctaactga agttcttttt aatgagaaga ttagagaaca gattaagaaa tacaggcgcc
961atttcctacg attttgtcac aacaacaaaa aagcccaacg gtaccttctt catggtttgg
1021agtgtgtggt agcaatgcat caagctcagc ttatctccaa gattccacat atcttgaagg
1081agatgtacga tgcagacctt ttagaagaag aggtcatcat cagctggtcg gaaaaggcct
1141ctaagaaata tgtctccaaa gaacttgcca aagagattcg tgtcaaagca gaaccattta
1201taaaatggtt gaaggaggca gaggaagaat cttctggtgg cgaagaagaa gatgaagatg
1261agaacattga ggtggtgtat tcgaaggctg ccagtgtacc gaaagttgag actgtaaagt
1321cagacaacaa ggatgacgac atcgatattg atgccattta cccaactttc ttgtacaaag
1381tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
1441gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta

Download FASTA (ORF) (Full)