Construct: ORF ccsbBroad304_06168

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015577.1_s304c1, BRDN0000462257
Derived from:
ccsbBroadEn_06168
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EPHA3 (2042)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06168
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2042 EPHA3 EPH receptor A3 NM_005233.6 99.9% 99.7% 1822G>A;2059G>A
2 human 2042 EPHA3 EPH receptor A3 XM_005264715.3 99.8% 99.6% 1429_1430insAGC;1819G>A;2056G>A
3 human 2042 EPHA3 EPH receptor A3 XM_005264716.3 93.2% 92.9% (many diffs)
4 human 2042 EPHA3 EPH receptor A3 NM_182644.3 54.6% 53.9% (many diffs)
5 mouse 13837 Epha3 Eph receptor A3 NM_010140.3 88.7% 96.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3015
ORF length:
2949
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga ttgtcagctc tccatcctcc tccttctcag ctgctctgtt ctcgacagct
121tcggggaact gattccgcag ccttccaatg aagtcaatct actggattca aaaacaattc
181aaggggagct gggctggatc tcttatccat cacatgggtg ggaagagatc agtggtgtgg
241atgaacatta cacacccatc aggacttacc aggtgtgcaa tgtcatggac cacagtcaaa
301acaattggct gagaacaaac tgggtcccca ggaactcagc tcagaagatt tatgtggagc
361tcaagttcac tctacgagac tgcaatagca ttccattggt tttaggaact tgcaaggaga
421cattcaacct gtactacatg gagtctgatg atgatcatgg ggtgaaattt cgagagcatc
481agtttacaaa gattgacacc attgcagctg atgaaagttt cactcaaatg gatcttgggg
541accgtattct gaagctcaac actgagatta gagaagtagg tcctgtcaac aagaagggat
601tttatttggc atttcaagat gttggtgctt gtgttgcctt ggtgtctgtg agagtatact
661tcaaaaagtg cccatttaca gtgaagaatc tggctatgtt tccagacacg gtacccatgg
721actcccagtc cctggtggag gttagagggt cttgtgtcaa caattctaag gaggaagatc
781ctccaaggat gtactgcagt acagaaggcg aatggcttgt acccattggc aagtgttcct
841gcaatgctgg ctatgaagaa agaggtttta tgtgccaagc ttgtcgacca ggtttctaca
901aggcattgga tggtaatatg aagtgtgcta agtgcccgcc tcacagttct actcaggaag
961atggttcaat gaactgcagg tgtgagaata attacttccg ggcagacaaa gaccctccat
1021ccatggcttg tacccgacct ccatcttcac caagaaatgt tatctctaat ataaacgaga
1081cctcagttat cctggactgg agttggcccc tggacacagg aggccggaaa gatgttacct
1141tcaacatcat atgtaaaaaa tgtgggtgga atataaaaca gtgtgagcca tgcagcccaa
1201atgtccgctt cctccctcga cagtttggac tcaccaacac cacggtgaca gtgacagacc
1261ttctggcaca tactaactac acctttgaga ttgatgccgt taatggggtg tcagagctga
1321gctccccacc aagacagttt gctgcggtca gcatcacaac taatcaggct gctccatcac
1381ctgtcctgac gattaagaaa gatcggacct ccagaaatag catctctttg tcctggcaag
1441aacctgaaca tcctaatggg atcatattgg actacgaggt caaatactat gaaaagcagg
1501aacaagaaac aagttatacc attctgaggg caagaggcac aaatgttacc atcagtagcc
1561tcaagcctga cactatatac gtattccaaa tccgagcccg aacagccgct ggatatggga
1621cgaacagccg caagtttgag tttgaaacta gtccagactc tttctccatc tctggtgaaa
1681gtagccaagt ggtcatgatc gccatttcag cggcagtagc aattattctc ctcactgttg
1741tcatctatgt tttgattggg aggttctgtg gctataagtc aaaacatggg gcagatgaaa
1801aaagacttca ttttggcaat gggcatttaa aacttccagg tctcaggact tatgttgacc
1861cacatacata tgaagaccct acccaaactg ttcatgagtt tgccaaggaa ttggatgcca
1921ccaacatatc cattgataaa gttgttggag caggtgaatt tggagaggtg tgcagtggtc
1981gcttaaaact tccttcaaaa aaagagattt cagtggccat taagaccctg aaagttggct
2041acacagaaaa gcagaggaga gacttcctgg gagaagcaag cattatggga cagtttgacc
2101accccaatat cattcgactg gaaagagttg ttaccaaaag taagccagtt atgattgtca
2161cagaatacat ggagaatggt tccttggata gtttcctacg taaacacgat gcccagttta
2221ctgtcattca gctagtgggg atgcttcgag ggatagcatc tggcatgaag tacctgtcag
2281acatgggcta tgttcaccga gacctcgctg ctcggaacat cttgatcaac agtaacttgg
2341tgtgtaaggt ttctgatttc ggactttcgc gtgtcctgga ggatgaccca gaagctgctt
2401atacaacaag aggagggaag atcccaatca ggtggacatc accagaagct atagcctacc
2461gcaagttcac gtcagccagc gatgtatgga gttatgggat tgttctctgg gaggtgatgt
2521cttatggaga gagaccatac tgggagatgt ccaatcagga tgtaattaaa gctgtagatg
2581agggctatcg actgccaccc cccatggact gcccagctgc cttgtatcag ctgatgctgg
2641actgctggca gaaagacagg aacaacagac ccaagtttga gcagattgtt agtattctgg
2701acaagcttat ccggaatccc ggcagcctga agatcatcac cagtgcagcc gcaaggccat
2761caaaccttct tctggaccaa agcaatgtgg atatcactac cttccgcaca acaggtgact
2821ggcttaatgg tgtctggaca gcacactgca aggaaatctt cacgggtgtg gagtacagtt
2881cttgtgacac aatagccaag atttccacag atgacatgaa aaaggttggt gtcaccgtgg
2941ttgggccaca gaagaagatc atcagtagca ttaaagctct agaaacgcaa tcaaagaatg
3001gcccagttcc cgtgtaccca actttcttgt acaaagtggt tggtaagcct atccctaacc
3061ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
3121cgacaatcaa cctctggatt a

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