Construct: ORF ccsbBroad304_06193

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009720.1_s304c1, BRDN0000462051
Derived from:
ccsbBroadEn_06193
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ACSL3 (2181)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06193
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2181 ACSL3 acyl-CoA synthetase long ch... NM_001354158.2 99.9% 99.8% 11A>T;1620C>T
2 human 2181 ACSL3 acyl-CoA synthetase long ch... NM_001354159.2 99.9% 99.8% 11A>T;1620C>T
3 human 2181 ACSL3 acyl-CoA synthetase long ch... NM_004457.5 99.9% 99.8% 11A>T;1620C>T
4 human 2181 ACSL3 acyl-CoA synthetase long ch... NM_203372.3 99.9% 99.8% 11A>T;1620C>T
5 mouse 74205 Acsl3 acyl-CoA synthetase long-ch... NM_001033606.2 88.8% 92.7% (many diffs)
6 mouse 74205 Acsl3 acyl-CoA synthetase long-ch... NM_028817.3 88.8% 92.7% (many diffs)
7 mouse 74205 Acsl3 acyl-CoA synthetase long-ch... NM_001136222.1 70.4% 74.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2229
ORF length:
2160
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcagcat gaataacctc gtgtcttcaa aaccatctac catgaagcta aaacatacca
121tcaaccctat tcttttatat tttatacatt ttctaatatc actttatact attttaacat
181acattccgtt ttattttttc tccgagtcaa gacaagaaaa atcaaaccga attaaagcaa
241agcctgtaaa ttcaaaacct gattctgcat acagatctgt taatagtttg gatggtttgg
301cttcagtatt ataccctgga tgtgatactt tagataaagt ttttacatat gcaaaaaaca
361aatttaagaa caaaagactc ttgggaacac gtgaagtttt aaatgaggaa gatgaagtac
421aaccaaatgg aaaaattttt aaaaaggtta ttcttggaca gtataattgg ctttcctatg
481aagatgtctt tgttcgagcc tttaattttg gaaatggatt acagatgttg ggtcagaaac
541caaagaccaa catcgccatc ttctgtgaga ccagggccga gtggatgata gctgcacagg
601cgtgttttat gtataatttt cagcttgtta cattatatgc cactctagga ggtccagcca
661ttgttcatgc attaaatgaa acagaggtga ccaacatcat tactagtaaa gaactcttac
721aaacaaagtt gaaggatata gtttctttgg tcccacgcct gcggcacatc atcactgttg
781atggaaagcc accgacctgg tccgagttcc ccaagggcat cattgtgcat accatggctg
841cagtggaggc cctgggagcc aaggccagca tggaaaacca acctcatagc aaaccattgc
901cctcagatat tgcagtaatc atgtacacaa gtggatccac aggacttcca aagggagtca
961tgatctcaca tagtaacatt attgctggta taactgggat ggcagaaagg attccagaac
1021taggagagga agatgtctac attggatatt tgcctctggc ccatgttcta gaattaagtg
1081ctgagcttgt ctgtctttct cacggatgcc gcattggtta ctcttcacca cagactttag
1141cagatcagtc ttcaaaaatt aaaaaaggaa gcaaagggga tacatccatg ttgaaaccaa
1201cactgatggc agcagttccg gaaatcatgg atcggatcta caaaaatgtc atgaataaag
1261tcagtgaaat gagtagtttt caacgtaatc tgtttattct ggcctataat tacaaaatgg
1321aacagatttc aaaaggacgt aatactccac tgtgcgacag ctttgttttc cggaaagttc
1381gaagcttgct agggggaaat attcgtctcc tgttgtgtgg tggcgctcca ctttctgcaa
1441ccacgcagcg attcatgaac atctgtttct gctgtcctgt tggtcaggga tacgggctca
1501ctgaatctgc tggggctgga acaatttccg aagtgtggga ctacaatact ggcagagtgg
1561gagcaccatt agtttgctgt gaaatcaaat taaaaaactg ggaggaaggt ggatacttta
1621atactgataa gccacacccc aggggtgaaa ttcttattgg gggccaaagt gtgacaatgg
1681ggtactataa aaatgaagca aaaacaaaag ctgatttctt tgaagatgaa aatggacaaa
1741ggtggctctg tactggggat attggagagt ttgaacccga tggatgctta aagattattg
1801atcgtaaaaa ggaccttgta aaactacagg caggggaata tgtttctctt gggaaagtag
1861aggcagcttt gaagaatctt ccactagtag ataacatttg tgcatatgca aacagttatc
1921attcttatgt cattggattt gttgtgccaa atcaaaagga actaactgaa ctagctcgaa
1981agaaaggact taaagggact tgggaggagc tgtgtaacag ttgtgaaatg gaaaatgagg
2041tacttaaagt gctttccgaa gctgctattt cagcaagtct ggaaaagttt gaaattccag
2101taaaaattcg tttgagtcct gaaccgtgga cccctgaaac tggtctggtg acagatgcct
2161tcaagctgaa acgcaaagag cttaaaacac attaccaggc ggacattgag cgaatgtatg
2221gaagaaaatt gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc
2281tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa
2341tcaacctctg gatta

Download FASTA (ORF) (Full)