Transcript: Mouse NM_028817.3

Mus musculus acyl-CoA synthetase long-chain family member 3 (Acsl3), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-21
Taxon:
Mus musculus (mouse)
Gene:
Acsl3 (74205)
Length:
3950
CDS:
460..2622

Additional Resources:

NCBI RefSeq record:
NM_028817.3
NBCI Gene record:
Acsl3 (74205)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_028817.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000369951 TTAACTGACAGGATTAGTAAA pLKO_005 2756 3UTR 100% 13.200 18.480 N ACSL3 n/a
2 TRCN0000076057 CTGCGGCATATCATTACTGTT pLKO.1 1150 CDS 100% 4.950 6.930 N Acsl3 n/a
3 TRCN0000076053 GCAAACTCCATTCCTCATATT pLKO.1 2699 3UTR 100% 13.200 9.240 N Acsl3 n/a
4 TRCN0000045528 GCCCTCAGATATTGCAGTAAT pLKO.1 1290 CDS 100% 13.200 9.240 N ACSL3 n/a
5 TRCN0000300534 GCCCTCAGATATTGCAGTAAT pLKO_005 1290 CDS 100% 13.200 9.240 N ACSL3 n/a
6 TRCN0000076056 GCCTTCAAGTTGAAACGTAAA pLKO.1 2548 CDS 100% 10.800 7.560 N Acsl3 n/a
7 TRCN0000076055 CACCATTAGTTTGCTGTGAAA pLKO.1 1955 CDS 100% 4.950 3.465 N Acsl3 n/a
8 TRCN0000076054 CGTATGCAAACAGCTACCATT pLKO.1 2294 CDS 100% 4.950 3.465 N Acsl3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_028817.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06193 pDONR223 100% 88.8% 92.7% None (many diffs) n/a
2 ccsbBroad304_06193 pLX_304 0% 88.8% 92.7% V5 (many diffs) n/a
3 TRCN0000475652 CTGACTCCCATGGCAATGATCAGT pLX_317 13.9% 88.8% 92.7% V5 (many diffs) n/a
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