Construct: ORF ccsbBroad304_06195
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015128.1_s304c1, BRDN0000462391
- Derived from:
- ccsbBroadEn_06195
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FBLN2 (2199)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06195
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2199 | FBLN2 | fibulin 2 | NM_001998.3 | 99.8% | 99.8% | (many diffs) |
| 2 | human | 2199 | FBLN2 | fibulin 2 | NM_001004019.2 | 96% | 96% | (many diffs) |
| 3 | human | 2199 | FBLN2 | fibulin 2 | NM_001165035.2 | 96% | 96% | (many diffs) |
| 4 | human | 2199 | FBLN2 | fibulin 2 | XM_006713026.2 | 92.9% | 92.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3618
- ORF length:
- 3552
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt gctgctctgg gagcctgcag gagcctggct tgctctgggc ctggccctgg 121 ccctgggccc cagcgtggcc gcagctgccc ctcggcagga ctgcacgggc gtggagtgcc 181 cgccgctgga gaactgcatt gaggaggcgc tggagccggg tgcctgctgt gccacgtgtg 241 tgcagcaggg ctgcgcctgc gagggctacc agtactatga ctgcctacag ggtggcttcg 301 tgcgcggccg cgtgcccgcc ggtcagtcct attttgtgga cttcgggagc actgagtgct 361 cctgcccacc aggcggcggc aagatcagct gccagttcat gctgtgcccg gagctgccgc 421 ccaactgcat cgaggctgta gtggtggctg acagctgccc acagtgcggc caggtgggct 481 gcgtccacgc gggccacaag tacgccgctg gccacactgt tcacctgccg ccctgccggg 541 cctgccactg ccctgacgcc ggtggagagc tcatctgcta ccagctcccc ggttgccacg 601 ggaacttctc agatgccgag gagggtgacc ccgagcgaca ctacgaagac ccctacagct 661 atgaccagga ggtggccgag gtggaagcag caacagccct ggggggtgag gtccaggcgg 721 gtgcagtcca ggcaggcgca gggggccccc cagctgctct gggaggtggg agtcagccac 781 tgtccaccat ccaggcaccc ccctggccag ctgtcctccc caggcccaca gcggctgctg 841 ccctgggtcc cccagcccca gtgcaggcca aagctaggag agtgaccgag gacagtgagg 901 aggaagaaga ggaggaggag gagagagagg aaatggctgt cactgagcag ctggcagcag 961 gtggccacag ggggctggat gggctgccca ctacagcccc agctggaccc agtcttccta 1021 tccaggagga gagggcagaa gctggggcaa gggcagaagc tggggcaagg cctgaagaga 1081 acctcatcct ggatgcccaa gccacgtccc gcagcactgg gccggagggc gtgacgcatg 1141 caccgagcct gggcaaggct gctctcgtcc caactcaggc cgtgcctggc tctcccaggg 1201 acccagtcaa gcccagcccc cacaacatcc tgtccacatc actgcctgat gcagcctgga 1261 tcccacccac ccgagaagtg cccaggaagc cgcaagttct gccccattcc cacgtggagg 1321 aggacacaga ccccaactct gtccattcta tccccagaag tagccctgaa ggctccacca 1381 aggacctgat cgagacttgc tgcgcagccg gacagcagtg ggccattgac aatgacgagt 1441 gcctggagat ccctgagagt ggcactgagg acaacgtctg caggacagcc cagaggcact 1501 gctgtgtctc ctacttgcag gagaagagct gcatggccgg cgtcctggga gccaaggagg 1561 gtgagacctg tggggctgag gacaacgaca gctgcggcat ctccctgtac aagcaatgct 1621 gtgactgctg tggcctgggc ctccgcgtgc gggccgaggg ccagtcgtgt gagtccaatc 1681 ctaacctggg ctatccctgc aatcatgtca tgctctcctg ctgtgagggt gaagagcctc 1741 tcatagtacc tgaggttcgc cgacctccag agcccgcagc tgcaccacgg agagtttcag 1801 aggcagagat ggcgggccga gaggccctgt cactgggcac agaggccgag ctgccgaaca 1861 gcctgccggg cgatgaccag gatgagtgcc ttctcctccc gggagagctg tgccagcacc 1921 tttgcatcaa tactgtgggt tcttaccact gtgcctgctt tcctggcttc tcactgcagg 1981 acgatggccg cacttgccgc ccagagggtc accctccaca gccggaagcc ccacaggagc 2041 ctgcactgaa gtcagaattt tcccaggtgg cctctaacac catcccgctg ccactgccgc 2101 agcccaatac ctgcaaagac aatggaccct gcaagcaggt gtgcagcact gttgggggct 2161 cagccatatg ctcctgtttt cccggctatg ccatcatggc ggatggcgtg tcctgtgaag 2221 acatcaacga gtgtgtgacg gacctgcaca cgtgcagccg gggcgagcac tgtgtgaaca 2281 cactgggctc cttccactgc tacaaggcac tcacctgtga gccaggctat gccctcaagg 2341 atggcgagtg cgaagacgtg gatgagtgtg cgatgggcac gcacacctgc cagccgggct 2401 tcttgtgcca gaacaccaag ggctccttct actgccaggc caggcagcgc tgcatggatg 2461 gcttcctgca ggatcctgaa ggcaactgtg tggacatcaa cgagtgcacg tcactgtccg 2521 agccatgtcg gccaggcttc agctgcatca acacggtggg ctcctacacg tgccagagga 2581 acccgctgat ctgcgcgcgc ggctaccacg ccagcgatga tggggccaag tgtgtggacg 2641 tgaatgagtg tgagacaggt gtgcaccgct gcggtgaggg ccaagtgtgc cacaacctcc 2701 ctggctccta ccgctgtgac tgcaaagccg gctttcagcg ggatgccttc ggccggggct 2761 gcatcgacgt gaatgagtgc tgggcctcgc caggccgcct gtgccagcac acgtgtgaga 2821 acacactcgg ctcctaccgc tgttcctgcg cctccgggtt cctgctagca gcggacggca 2881 agcgctgtga agacgtgaat gagtgtgagg cccagcgctg cagccaggag tgtgccaaca 2941 tctatggctc ctaccagtgc tactgccgcc agggctacca gctggctgag gatgggcaca 3001 cctgcacaga catcgacgag tgtgctcaag gcgccggcat cctctgcacc ttccgctgtc 3061 tcaacgtgcc agggagctac cagtgtgcat gccctgagca gggctacacc atgacggcca 3121 acgggaggtc ctgcaaggac gtggatgagt gtgcactggg tacccacaac tgttccgagg 3181 ctgagacctg ccacaacatc cagggtagct tccgctgcct gcgcttagag tgtcctccca 3241 actatgtcca agtctccaaa acgaagtgcg agcgcaccac gtgccatgac ttcctggagt 3301 gccagaactc gccagcgcgc atcacgcact accagctcaa cttccagacg ggcctcctgg 3361 tgcctgcgca tatcttccgc attggccccg cgccagcctt cacgggggac accatcgccc 3421 tgaacatcat caagggcaat gaggagggct actttggcac gcgcaggctc aatgcctaca 3481 cgggtgtggt ctacctgcag cgggccgtgc tggagccccg ggactttgcc ctggacgtgg 3541 agatgaagct ctggaggcag ggctccgtca ccaccttcct ggccaagatg cacatcttct 3601 tcaccacctt tgccctgtgc ccaactttct tgtacaaagt ggttggtaag cctatcccta 3661 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 3721 agtcgacaat caacctctgg atta