Construct: ORF ccsbBroad304_06218
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004140.1_s304c1, BRDN0000400247
- Derived from:
- ccsbBroadEn_06218
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FMO3 (2328)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06218
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2328 | FMO3 | flavin containing dimethyla... | NM_001002294.3 | 99.9% | 99.8% | 769G>A |
| 2 | human | 2328 | FMO3 | flavin containing dimethyla... | NM_006894.6 | 99.9% | 99.8% | 769G>A |
| 3 | human | 2328 | FMO3 | flavin containing dimethyla... | NM_001319173.2 | 93.8% | 92.7% | (many diffs) |
| 4 | human | 2328 | FMO3 | flavin containing dimethyla... | XM_011509345.3 | 93.8% | 92.7% | (many diffs) |
| 5 | human | 2328 | FMO3 | flavin containing dimethyla... | NM_001319174.2 | 88% | 87.9% | 132_133ins189;580G>A |
| 6 | human | 2328 | FMO3 | flavin containing dimethyla... | XM_024454365.1 | 53.1% | 48.6% | 0_1ins547;79_80ins200 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1662
- ORF length:
- 1596
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg gaagaaagtg gccatcattg gagctggtgt gagtggcttg gcctccatca 121 ggagctgtct ggaagagggg ctggagccca cctgctttga gaagagcaat gacattgggg 181 gcctgtggaa attttcagac catgcagagg agggcagggc tagcatttac aaatcagtct 241 tttccaactc ttccaaagag atgatgtgtt tcccagactt cccatttccc gatgacttcc 301 ccaactttat gcacaacagc aagatccagg aatatatcat tgcatttgcc aaagaaaaga 361 acctcctgaa gtacatacaa tttaagacat ttgtatccag tgtaaataaa catcctgatt 421 ttgcaactac tggccagtgg gatgttacca ctgaaaggga tggtaaaaaa gaatcggctg 481 tctttgatgc tgtaatggtt tgttccggac atcatgtgta tcccaaccta ccaaaagagt 541 cctttccagg actaaaccac tttaaaggca aatgcttcca cagcagggac tataaagaac 601 caggtgtatt caatggaaag cgtgtcctgg tggttggcct ggggaattcg ggctgtgata 661 ttgccacaga actcagccgc acagcagaac aggtcatgat cagttccaga agtggctcct 721 gggtgatgag ccgggtctgg gacaatggtt atccttggga catgctgctc gtcactcgat 781 ttggaacctt cctcaagaac aatttaccga cagccatctc tgactggttg tacatgaagc 841 agatgaatgc aagattcaag catgaaaact atggcttgat gcctttaaat ggagtcctga 901 ggaaagagcc tgtatttaac gatgagctcc cagcaagcat tctgtgtggc attgtgtccg 961 taaagcctaa cgtgaaggaa ttcacagaga cctcggccat ttttgaggat gggaccatat 1021 ttgagggcat tgactgtgta atctttgcaa cagggtatag ttttgcctac cccttccttg 1081 atgagtctat catcaaaagc agaaacaatg agatcatttt atttaaagga gtatttcctc 1141 ctctacttga gaagtcaacc atagcagtga ttggctttgt ccagtccctt ggggctgcca 1201 ttcccacagt tgacctccag tcccgctggg cagcacaagt aataaaggga acttgtactt 1261 tgccttctat ggaagacatg atgaatgata ttaatgagaa aatggagaaa aagcgcaaat 1321 ggtttggcaa aagcgagacc atacagacag attacattgt ttatatggat gaactctcct 1381 ccttcattgg ggcaaagccc aacatcccat ggctgtttct cacagatccc aaattggcca 1441 tggaagttta ttttggccct tgtagtccct accagtttag gctggtgggc ccagggcagt 1501 ggccaggagc cagaaatgcc atactgaccc agtgggaccg gtcgttgaaa cccatgcaga 1561 cacgagtggt cgggagactt cagaagcctt gcttcttttt ccattggctg aagctctttg 1621 caattcctat tctgttaatc gctgttttcc ttgtgttgac ctacccaact ttcttgtaca 1681 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 1741 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta