Construct: ORF ccsbBroad304_06245

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001993.1_s304c1, BRDN0000403592
Derived from:
ccsbBroadEn_06245
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GABRG1 (2565)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06245
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2565 GABRG1 gamma-aminobutyric acid typ... NM_173536.4 99.8% 99.5% 1208G>A;1391A>C
2 human 2565 GABRG1 gamma-aminobutyric acid typ... XM_017007990.2 72.1% 71.8% 0_1ins387;821G>A;1004A>C
3 mouse 14405 Gabrg1 gamma-aminobutyric acid (GA... NM_010252.4 87.5% 94.6% (many diffs)
4 mouse 14405 Gabrg1 gamma-aminobutyric acid (GA... XM_006503737.2 76.8% 74.9% (many diffs)
5 mouse 14405 Gabrg1 gamma-aminobutyric acid (GA... XM_011240701.2 73.8% 75.8% (many diffs)
6 mouse 14405 Gabrg1 gamma-aminobutyric acid (GA... XM_006503738.1 71.8% 77.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1461
ORF length:
1395
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggg tcctttgaaa gcttttctct tctccccttt tcttctgcgg agtcaaagta
121gaggggtgag gttggtcttc ttgttactga ccctgcattt gggaaactgt gttgataagg
181cagatgatga agatgatgag gatttaacgg tgaacaaaac ctgggtcttg gccccaaaaa
241ttcatgaagg agatatcaca caaattctga attcattgct tcaaggctat gacaataaac
301ttcgtccaga tataggagtg aggcccacag taattgaaac tgatgtttat gtaaacagca
361ttggaccagt tgatccaatt aatatggaat atacaataga tataattttt gcccaaacct
421ggtttgacag tcgtttaaaa ttcaatagta ccatgaaagt gcttatgctt aacagtaata
481tggttggaaa aatttggatt cctgacactt tcttcagaaa ctcaagaaaa tctgatgctc
541actggataac aactcctaat cgtctgcttc gaatttggaa tgatggacga gttctgtata
601ctctaagatt gacaattaat gcagaatgtt atcttcagct tcataacttt cccatggatg
661aacattcctg tccactggaa ttttcaagct atggataccc taaaaatgaa attgagtata
721agtggaaaaa gccctccgta gaagtggctg atcctaaata ctggagatta tatcagtttg
781catttgtagg gttacggaac tcaactgaaa tcactcacac gatctctggg gattatgtta
841tcatgacaat tttttttgac ctgagcagaa gaatgggata tttcactatt cagacctaca
901ttccatgcat tctgacagtt gttctttctt gggtgtcttt ttggatcaat aaagatgcag
961tgcctgcaag aacatcgttg ggtatcacta cagttctgac tatgacaacc ctgagtacaa
1021ttgccaggaa gtctttacct aaggtttctt atgtgactgc gatggatctc tttgtttctg
1081tttgtttcat ttttgttttt gcagccttga tggaatatgg aaccttgcat tattttacca
1141gcaaccaaaa aggaaagact gctactaaag acagaaagct aaaaaataaa gcctcgatga
1201ctcctggtct ccatcctgga tccactctga ttccaatgaa taatatttct gtgccgcaag
1261aagatgatta tgagtatcag tgtttggagg gcaaagattg tgccagcttc ttctgttgct
1321ttgaagactg cagaacagga tcttggaggg aaggaaggat acacatacgc attgccaaaa
1381ttgactctta ttctagaata tttttcccaa ccgcttttgc cctgttcaac ttggtttatt
1441gggttggcta tctttcctta tacccaactt tcttgtacaa agtggttggt aagcctatcc
1501ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
1561ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)