Construct: ORF ccsbBroad304_06245
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001993.1_s304c1, BRDN0000403592
- Derived from:
- ccsbBroadEn_06245
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GABRG1 (2565)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06245
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2565 | GABRG1 | gamma-aminobutyric acid typ... | NM_173536.4 | 99.8% | 99.5% | 1208G>A;1391A>C |
| 2 | human | 2565 | GABRG1 | gamma-aminobutyric acid typ... | XM_017007990.2 | 72.1% | 71.8% | 0_1ins387;821G>A;1004A>C |
| 3 | mouse | 14405 | Gabrg1 | gamma-aminobutyric acid (GA... | NM_010252.4 | 87.5% | 94.6% | (many diffs) |
| 4 | mouse | 14405 | Gabrg1 | gamma-aminobutyric acid (GA... | XM_006503737.2 | 76.8% | 74.9% | (many diffs) |
| 5 | mouse | 14405 | Gabrg1 | gamma-aminobutyric acid (GA... | XM_011240701.2 | 73.8% | 75.8% | (many diffs) |
| 6 | mouse | 14405 | Gabrg1 | gamma-aminobutyric acid (GA... | XM_006503738.1 | 71.8% | 77.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1461
- ORF length:
- 1395
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg tcctttgaaa gcttttctct tctccccttt tcttctgcgg agtcaaagta 121 gaggggtgag gttggtcttc ttgttactga ccctgcattt gggaaactgt gttgataagg 181 cagatgatga agatgatgag gatttaacgg tgaacaaaac ctgggtcttg gccccaaaaa 241 ttcatgaagg agatatcaca caaattctga attcattgct tcaaggctat gacaataaac 301 ttcgtccaga tataggagtg aggcccacag taattgaaac tgatgtttat gtaaacagca 361 ttggaccagt tgatccaatt aatatggaat atacaataga tataattttt gcccaaacct 421 ggtttgacag tcgtttaaaa ttcaatagta ccatgaaagt gcttatgctt aacagtaata 481 tggttggaaa aatttggatt cctgacactt tcttcagaaa ctcaagaaaa tctgatgctc 541 actggataac aactcctaat cgtctgcttc gaatttggaa tgatggacga gttctgtata 601 ctctaagatt gacaattaat gcagaatgtt atcttcagct tcataacttt cccatggatg 661 aacattcctg tccactggaa ttttcaagct atggataccc taaaaatgaa attgagtata 721 agtggaaaaa gccctccgta gaagtggctg atcctaaata ctggagatta tatcagtttg 781 catttgtagg gttacggaac tcaactgaaa tcactcacac gatctctggg gattatgtta 841 tcatgacaat tttttttgac ctgagcagaa gaatgggata tttcactatt cagacctaca 901 ttccatgcat tctgacagtt gttctttctt gggtgtcttt ttggatcaat aaagatgcag 961 tgcctgcaag aacatcgttg ggtatcacta cagttctgac tatgacaacc ctgagtacaa 1021 ttgccaggaa gtctttacct aaggtttctt atgtgactgc gatggatctc tttgtttctg 1081 tttgtttcat ttttgttttt gcagccttga tggaatatgg aaccttgcat tattttacca 1141 gcaaccaaaa aggaaagact gctactaaag acagaaagct aaaaaataaa gcctcgatga 1201 ctcctggtct ccatcctgga tccactctga ttccaatgaa taatatttct gtgccgcaag 1261 aagatgatta tgagtatcag tgtttggagg gcaaagattg tgccagcttc ttctgttgct 1321 ttgaagactg cagaacagga tcttggaggg aaggaaggat acacatacgc attgccaaaa 1381 ttgactctta ttctagaata tttttcccaa ccgcttttgc cctgttcaac ttggtttatt 1441 gggttggcta tctttcctta tacccaactt tcttgtacaa agtggttggt aagcctatcc 1501 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 1561 ttaagtcgac aatcaacctc tggatta