Construct: ORF ccsbBroad304_06266

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009805.1_s304c1, BRDN0000408245
Derived from:
ccsbBroadEn_06266
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GCKR (2646)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06266
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2646 GCKR glucokinase regulator NM_001486.4 99.9% 99.8% 1337T>C
2 human 2646 GCKR glucokinase regulator XM_017003796.1 69.5% 69.4% 0_1ins570;767T>C
3 human 2646 GCKR glucokinase regulator XM_017003797.1 69.5% 69.4% 0_1ins570;767T>C
4 human 2646 GCKR glucokinase regulator XR_001738699.1 59.6% 1_66del;1029_1030ins175;1223_1224ins543
5 human 2646 GCKR glucokinase regulator XM_011532763.1 53.2% 51.8% 967_968ins98;999_1000ins778
6 mouse 231103 Gckr glucokinase regulatory protein XM_006503881.3 85.2% 87.4% (many diffs)
7 mouse 231103 Gckr glucokinase regulatory protein XM_006503882.3 82.7% 84.7% (many diffs)
8 mouse 231103 Gckr glucokinase regulatory protein NM_144909.1 80.4% 81.8% (many diffs)
9 mouse 231103 Gckr glucokinase regulatory protein XM_006503883.3 78.1% 80.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1944
ORF length:
1875
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gccaggcaca aaacggtttc aacatgtcat tgagaccccg gagcctggca
121agtgggagtt gtctgggtac gaggcagctg tgccaatcac ggagaagtca aacccactga
181cccaggatct agacaaagca gatgctgaga acattgttcg actgctaggg caatgtgatg
241ctgagatctt ccaggaggag gggcaagccc tgtccacata ccagagactc tacagcgaat
301ccattctgac caccatggta caggtggctg ggaaagttca ggaagtgctg aaggagccag
361atggggggct ggttgtgctg agtggagggg gcacctctgg ccggatggca ttcctcatgt
421cggtgtcctt taatcagctg atgaaaggtc tgggacagaa acctctttac acctacctca
481ttgcaggtgg tgacaggtct gtggtggcct ctagggaggg gacagaagat agtgccttgc
541acgggattga ggaactgaag aaggtggctg ccgggaagaa gagagtgatt gtcattggca
601tttctgtggg actctctgct ccctttgtgg caggccagat ggactgctgc atgaacaaca
661cagctgtctt cttgccagtc ctggttggct tcaatccagt gagcatggcc agaaatgacc
721ccattgaaga ctggagttca acattccgac aagtagcaga gcggatgcag aaaatgcagg
781agaaacagaa agcttttgtg ctcaatcctg ccatcgggcc cgagggtctc agcggctcct
841cccggatgaa aggtggaagt gccaccaaga ttctgctgga aaccctgtta ttagcagccc
901ataagactgt ggaccagggc attgcagcat ctcaaagatg cctcctggaa atcttgcgga
961catttgagcg agctcatcag gtgacctaca gccaaagccc caagattgcc accctgatga
1021agagtgtcag caccagtctg gagaagaaag gccacgtgta cctggttggc tggcagaccc
1081tgggcatcat tgccatcatg gatggagtag agtgcatcca cacctttggt gctgatttcc
1141gagatgtccg tggctttctc attggtgatc acagtgacat gtttaaccag aaggctgagc
1201tcaccaacca gggtccccag ttcaccttct cccaggagga cttcctgact tccatccttc
1261cctctctcac ggaaatcgat actgtggtct tcattttcac cctggatgac aacctcacgg
1321aggtgcagac tatagtggag caggtgaaag agaagaccaa ccacatccag gccctggcac
1381acagcaccgt gggtcagacc ttgccgatcc ctctgaagaa gctctttccc tccatcatca
1441gcatcacatg gccactgctt ttctttgaat atgaagggaa cttcatccag aagttccagc
1501gtgagctaag caccaaatgg gtgctgaata cagtgagtac aggtgctcat gtgcttcttg
1561gtaagatcct acaaaaccac atgttggacc ttcggattag caactccaag ctcttctggc
1621gggcgctggc catgctgcag cggttctctg gacagtccaa ggctcgatgc atcgagagcc
1681tcctccgagc gatccacttt ccccagccac tgtcagatga tattcgggct gctcccatct
1741cctgccatgt ccaggttgca catgagaagg aacaggtgat acccatcgcc ttgctgagcc
1801tcctattccg gtgctcgatc actgaggctc aggcacacct ggctgcagct ccttctgtct
1861gtgaggctgt caggagtgct cttgctgggc caggtcagaa gcgcactgcg gaccccctcg
1921agatcctaga gcctgacgtt cagttgccaa ctttcttgta caaagtggtt ggtaagccta
1981tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc
2041gcgttaagtc gacaatcaac ctctggatta

Download FASTA (ORF) (Full)