Construct: ORF ccsbBroad304_06280

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007434.1_s304c1, BRDN0000407931
Derived from:
ccsbBroadEn_06280
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GPC3 (2719)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06280
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2719 GPC3 glypican 3 NM_004484.4 99.9% 99.8% 1076C>T
2 human 2719 GPC3 glypican 3 NM_001164618.2 97.1% 96.8% 289_290ins48;1028C>T
3 human 2719 GPC3 glypican 3 NM_001164617.2 96.1% 96% 1033_1101del;1145C>T
4 human 2719 GPC3 glypican 3 NM_001164619.2 90.6% 90.5% 173_174ins162;914C>T
5 human 2719 GPC3 glypican 3 XM_017029413.2 78.5% 76.5% (many diffs)
6 mouse 14734 Gpc3 glypican 3 NM_016697.3 90.5% 93.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1806
ORF length:
1740
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc cgggaccgtg cgcaccgcgt gcttggtggt ggcgatgctg ctcagcttgg
121acttcccggg acaggcgcag cccccgccgc cgccgccgga cgccacctgt caccaagtcc
181gctccttctt ccagagactg cagcccggac tcaagtgggt gccagaaact cccgtgccag
241gatcagattt gcaagtatgt ctccctaagg gcccaacatg ctgctcaaga aagatggaag
301aaaaatacca actaacagca cgattgaaca tggaacagct gcttcagtct gcaagtatgg
361agctcaagtt cttaattatt cagaatgctg cggttttcca agaggccttt gaaattgttg
421ttcgccatgc caagaactac accaatgcca tgttcaagaa caactaccca agcctgactc
481cacaagcttt tgagtttgtg ggtgaatttt tcacagatgt gtctctctac atcttgggtt
541ctgacatcaa tgtagatgac atggtcaatg aattgtttga cagcctgttt ccagtcatct
601atacccagct aatgaaccca ggcctgcctg attcagcctt ggacatcaat gagtgcctcc
661gaggagcaag acgtgacctg aaagtatttg ggaatttccc caagcttatt atgacccagg
721tttccaagtc actgcaagtc actaggatct tccttcaggc tctgaatctt ggaattgaag
781tgatcaacac aactgatcac ctgaagttca gtaaggactg tggccgaatg ctcaccagaa
841tgtggtactg ctcttactgc cagggactga tgatggttaa accctgtggc ggttactgca
901atgtggtcat gcaaggctgt atggcaggtg tggtggagat tgacaagtac tggagagaat
961acattctgtc ccttgaagaa cttgtgaatg gcatgtacag aatctatgac atggagaacg
1021tactgcttgg tctcttttca acaatccatg attctatcca gtatgtccag aagaatgcag
1081gaaagctgac caccactatt ggcaagttat gtgcccattc tcaacaacgc caatatagat
1141ttgcttatta tcctgaagat ctctttattg acaagaaagt attaaaagtt gctcatgtag
1201aacatgaaga aaccttatcc agccgaagaa gggaactaat tcagaagttg aagtctttca
1261tcagcttcta tagtgctttg cctggctaca tctgcagcca tagccctgtg gcggaaaacg
1321acaccctttg ctggaatgga caagaactcg tggagagata cagccaaaag gcagcaagga
1381atggaatgaa aaaccagttc aatctccatg agctgaaaat gaagggccct gagccagtgg
1441tcagtcaaat tattgacaaa ctgaagcaca ttaaccagct cctgagaacc atgtctatgc
1501ccaaaggtag agttctggat aaaaacctgg atgaggaagg gtttgaaagt ggagactgcg
1561gtgatgatga agatgagtgc attggaggct ctggtgatgg aatgataaaa gtgaagaatc
1621agctccgctt ccttgcagaa ctggcctatg atctggatgt ggatgatgcg cctggaaaca
1681gtcagcaggc aactccgaag gacaacgaga taagcacctt tcacaacctc gggaacgttc
1741attccccgct gaagcttctc accagcatgg ccatctcggt ggtgtgcttc ttcttcctgg
1801tgcactgccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
1861gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
1921acctctggat ta

Download FASTA (ORF) (Full)