Construct: ORF ccsbBroad304_06337
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013301.1_s304c1, BRDN0000408109
- Derived from:
- ccsbBroadEn_06337
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GSTT2 (2953)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06337
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 653689 | GSTT2B | glutathione S-transferase t... | NM_001080843.3 | 99.8% | 100% | 543T>C |
| 2 | human | 2953 | GSTT2 | glutathione S-transferase t... | NM_000854.4 | 99.7% | 99.5% | 385A>G;543T>C |
| 3 | human | 653689 | GSTT2B | glutathione S-transferase t... | NM_001363804.1 | 94.1% | 94.2% | 479_480ins42;501T>C |
| 4 | human | 2953 | GSTT2 | glutathione S-transferase t... | NM_001302670.1 | 93.9% | 93.8% | 385A>G;479_480ins42;501T>C |
| 5 | human | 2953 | GSTT2 | glutathione S-transferase t... | NR_126445.2 | 65.7% | (many diffs) | |
| 6 | human | 2953 | GSTT2 | glutathione S-transferase t... | NR_126446.1 | 51.5% | (many diffs) | |
| 7 | human | 2953 | GSTT2 | glutathione S-transferase t... | NR_126442.1 | 46.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 798
- ORF length:
- 732
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 tcgccatggg cctagagctg tttcttgacc tggtgtccca gcccagccgc gccgtctaca 121 tcttcgccaa gaagaatggc atccccttag agctgcgcac cgtggatttg gtcaaagggc 181 agcacaagag caaggagttc ttgcagatca acagcctggg gaaactgccg acgctcaagg 241 atggtgattt catcttgacc gaaagctcgg ccatcctgat ttacctgagc tgtaagtacc 301 agacgccgga ccactggtat ccatctgacc tgcaggctcg tgcccgtgtt catgagtacc 361 tgggctggca tgccgactgc atccgtggca cctttggtat acccctgtgg gtccaggtgt 421 tggggccact cattggggtc caggtgcccg aggagaaggt ggaacgcaac aggactgcca 481 tggaccaggc cctgcaatgg ctggaggaca agttcctggg ggacaggccc ttcctcgctg 541 gccagcaggt gacactggct gatctcatgg ccctggagga gctgatgcag ccggtggctc 601 tcggctacga actgtttgag ggacggccac gactggcagc atggcgtgga cgagtggagg 661 ctttcctggg tgctgagcta tgccaggagg cccacagcat catcttgagc atcctggaac 721 aggcggccaa gaaaaccctc ccaacaccct caccagaggc ctatcaggct atgctgcttc 781 gaatcgccag gatcccctga agaactttct tgtacaaagt ggttggtaag cctatcccta 841 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 901 agtcgacaat caacctctgg atta