Construct: ORF ccsbBroad304_06414
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001359.1_s304c1, BRDN0000407107
- Derived from:
- ccsbBroadEn_06414
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HSPD1 (3329)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06414
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3329 | HSPD1 | heat shock protein family D... | NM_002156.5 | 99.8% | 100% | 27C>G;69T>C;273A>G |
| 2 | human | 3329 | HSPD1 | heat shock protein family D... | NM_199440.1 | 99.8% | 100% | 27C>G;69T>C;273A>G |
| 3 | mouse | 15510 | Hspd1 | heat shock protein 1 (chape... | NM_010477.4 | 90.6% | 97.5% | (many diffs) |
| 4 | mouse | 15510 | Hspd1 | heat shock protein 1 (chape... | XM_006495706.2 | 90.6% | 97.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1785
- ORF length:
- 1719
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct tcggttaccc acagtctttc ggcagatgag accggtgtcc agggtactgg 121 ctcctcatct cacccgggct tatgccaaag atgtaaaatt tggtgcagat gcccgagcct 181 taatgcttca aggtgtagac cttttagccg atgctgtggc cgttacaatg gggccaaagg 241 gaagaacagt gattattgag cagagttggg gaagtcccaa agtaacaaaa gatggtgtga 301 ctgttgcaaa gtcaattgac ttaaaagata aatacaagaa cattggagct aaacttgttc 361 aagatgttgc caataacaca aatgaagaag ctggggatgg cactaccact gctactgtac 421 tggcacgctc tatagccaag gaaggcttcg agaagattag caaaggtgct aatccagtgg 481 aaatcaggag aggtgtgatg ttagctgttg atgctgtaat tgctgaactt aaaaagcagt 541 ctaaacctgt gaccacccct gaagaaattg cacaggttgc tacgatttct gcaaacggag 601 acaaagaaat tggcaatatc atctctgatg caatgaaaaa agttggaaga aagggtgtca 661 tcacagtaaa ggatggaaaa acactgaatg atgaattaga aattattgaa ggcatgaagt 721 ttgatcgagg ctatatttct ccatacttta ttaatacatc aaaaggtcag aaatgtgaat 781 tccaggatgc ctatgttctg ttgagtgaaa agaaaatttc tagtatccag tccattgtac 841 ctgctcttga aattgccaat gctcaccgta agcctttggt cataatcgct gaagatgttg 901 atggagaagc tctaagtaca ctcgtcttga ataggctaaa ggttggtctt caggttgtgg 961 cagtcaaggc tccagggttt ggtgacaata gaaagaacca gcttaaagat atggctattg 1021 ctactggtgg tgcagtgttt ggagaagagg gattgaccct gaatcttgaa gacgttcagc 1081 ctcatgactt aggaaaagtt ggagaggtca ttgtgaccaa agacgatgcc atgctcttaa 1141 aaggaaaagg tgacaaggct caaattgaaa aacgtattca agaaatcatt gagcagttag 1201 atgtcacaac tagtgaatat gaaaaggaaa aactgaatga acggcttgca aaactttcag 1261 atggagtggc tgtgctgaag gttggtggga caagtgatgt tgaagtgaat gaaaagaaag 1321 acagagttac agatgccctt aatgctacaa gagctgctgt tgaagaaggc attgttttgg 1381 gagggggttg tgccctcctt cgatgcattc cagccttgga ctcattgact ccagctaatg 1441 aagatcaaaa aattggtata gaaattatta aaagaacact caaaattcca gcaatgacca 1501 ttgctaagaa tgcaggtgtt gaaggatctt tgatagttga gaaaattatg caaagttcct 1561 cagaagttgg ttatgatgct atggctggag attttgtgaa tatggtggaa aaaggaatca 1621 ttgacccaac aaaggttgtg agaactgctt tattggatgc tgctggtgtg gcctctctgt 1681 taactacagc agaagttgta gtcacagaaa ttcctaaaga agagaaggac cctggaatgg 1741 gtgcaatggg tggaatggga ggtggtatgg gaggtggcat gttctaccca actttcttgt 1801 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 1861 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a