Transcript: Human NM_199440.1

Homo sapiens heat shock protein family D (Hsp60) member 1 (HSPD1), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
HSPD1 (3329)
Length:
2319
CDS:
118..1839

Additional Resources:

NCBI RefSeq record:
NM_199440.1
NBCI Gene record:
HSPD1 (3329)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_199440.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000029445 CAGTGATTATTGAGCAGAGTT pLKO.1 299 CDS 100% 4.950 3.465 N HSPD1 n/a
2 TRCN0000343948 CAGTGATTATTGAGCAGAGTT pLKO_005 299 CDS 100% 4.950 3.465 N HSPD1 n/a
3 TRCN0000029448 GCTAAACTTGTTCAAGATGTT pLKO.1 400 CDS 100% 4.950 3.465 N HSPD1 n/a
4 TRCN0000343882 GCTAAACTTGTTCAAGATGTT pLKO_005 400 CDS 100% 4.950 3.465 N HSPD1 n/a
5 TRCN0000029444 CCAGCTTAAAGATATGGCTAT pLKO.1 1050 CDS 100% 4.050 2.835 N HSPD1 n/a
6 TRCN0000343951 CCAGCTTAAAGATATGGCTAT pLKO_005 1050 CDS 100% 4.050 2.835 N HSPD1 n/a
7 TRCN0000029446 CCTGCTCTTGAAATTGCCAAT pLKO.1 892 CDS 100% 4.050 2.835 N HSPD1 n/a
8 TRCN0000343883 CCTGCTCTTGAAATTGCCAAT pLKO_005 892 CDS 100% 4.050 2.835 N HSPD1 n/a
9 TRCN0000029447 GCAATGACCATTGCTAAGAAT pLKO.1 1543 CDS 100% 0.563 0.338 N HSPD1 n/a
10 TRCN0000343952 GCAATGACCATTGCTAAGAAT pLKO_005 1543 CDS 100% 0.563 0.338 N HSPD1 n/a
11 TRCN0000112195 CCATGCCTACAGATAATTTAT pLKO.1 2025 3UTR 100% 15.000 7.500 Y Hspd1 n/a
12 TRCN0000314434 CCATGCCTACAGATAATTTAT pLKO_005 2025 3UTR 100% 15.000 7.500 Y Hspd1 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_199440.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00800 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_00800 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000475088 GAAATATCTTTTCTGAAAAACAAA pLX_317 33.8% 100% 100% V5 n/a
4 ccsbBroadEn_15458 pDONR223 0% 99.8% 100% None 69T>C;273A>G n/a
5 ccsbBroad304_15458 pLX_304 0% 99.8% 100% V5 69T>C;273A>G n/a
6 TRCN0000471997 TGTTTTCTACCCCAACCACTCGCA pLX_317 22.8% 99.8% 100% V5 69T>C;273A>G n/a
7 ccsbBroadEn_06414 pDONR223 100% 99.8% 100% None 27C>G;69T>C;273A>G n/a
8 ccsbBroad304_06414 pLX_304 0% 99.8% 100% V5 27C>G;69T>C;273A>G n/a
Download CSV