Construct: ORF ccsbBroad304_06439
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004025.1_s304c1, BRDN0000460302
- Derived from:
- ccsbBroadEn_06439
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IL5 (3567)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06439
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3567 | IL5 | interleukin 5 | NM_000879.3 | 100% | 100% | |
| 2 | human | 3567 | IL5 | interleukin 5 | XM_011543373.3 | 100% | 100% | |
| 3 | human | 3567 | IL5 | interleukin 5 | XM_011543374.3 | 100% | 100% | |
| 4 | human | 3567 | IL5 | interleukin 5 | XM_005271988.4 | 85.8% | 85.8% | 1_66del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 471
- ORF length:
- 402
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaggatgctt ctgcatttga gtttgctagc tcttggagct gcctacgtgt 121 atgccatccc cacagaaatt cccacaagtg cattggtgaa agagaccttg gcactgcttt 181 ctactcatcg aactctgctg atagccaatg agactctgag gattcctgtt cctgtacata 241 aaaatcacca actgtgcact gaagaaatct ttcagggaat aggcacactg gagagtcaaa 301 ctgtgcaagg gggtactgtg gaaagactat tcaaaaactt gtccttaata aagaaataca 361 ttgacggcca aaaaaaaaag tgtggagaag aaagacggag agtaaaccaa ttcctagact 421 acctgcaaga gtttcttggt gtaatgaaca ccgagtggat aatagaaagt ttgccaactt 481 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 541 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta