Construct: ORF ccsbBroad304_06448
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003612.1_s304c1, BRDN0000407074
- Derived from:
- ccsbBroadEn_06448
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IL18 (3606)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06448
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3606 | IL18 | interleukin 18 | NM_001562.4 | 99.8% | 100% | 105A>C |
| 2 | human | 3606 | IL18 | interleukin 18 | XM_011542806.2 | 99.8% | 100% | 105A>C |
| 3 | human | 3606 | IL18 | interleukin 18 | NM_001243211.2 | 97.7% | 97.9% | 78_79insGCTGAAGATGAT;93A>C |
| 4 | human | 3606 | IL18 | interleukin 18 | XM_011542805.1 | 97.7% | 97.9% | 78_79insGCTGAAGATGAT;93A>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 645
- ORF length:
- 579
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tgctgaacca gtagaagaca attgcatcaa ctttgtggca atgaaattta 121 ttgacaatac gctttacttt atagctgaag atgatgaaaa cctggaatcc gattactttg 181 gcaagcttga atctaaatta tcagtcataa gaaatttgaa tgaccaagtt ctcttcattg 241 accaaggaaa tcggcctcta tttgaagata tgactgattc tgactgtaga gataatgcac 301 cccggaccat atttattata agtatgtata aagatagcca gcctagaggt atggctgtaa 361 ctatctctgt gaagtgtgag aaaatttcaa ctctctcctg tgagaacaaa attatttcct 421 ttaaggaaat gaatcctcct gataacatca aggatacaaa aagtgacatc atattctttc 481 agagaagtgt cccaggacat gataataaga tgcaatttga atcttcatca tacgaaggat 541 actttctagc ttgtgaaaaa gagagagacc tttttaaact cattttgaaa aaagaggatg 601 aattggggga tagatctata atgttcactg ttcaaaacga agactaccca actttcttgt 661 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 721 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a