Construct: ORF ccsbBroad304_06452

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009329.1_s304c1, BRDN0000405257
Derived from:
ccsbBroadEn_06452
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
IMPG1 (3617)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06452
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3617 IMPG1 interphotoreceptor matrix p... NM_001563.4 99.9% 99.7% 1552C>G;2110C>T
2 human 3617 IMPG1 interphotoreceptor matrix p... NM_001282368.1 90.1% 89.9% 65_66ins234;1318C>G;1876C>T
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2460
ORF length:
2391
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gtatttggaa actagaagag ctatttttgt tttttggatt tttctccaag
121ttcaaggaac caaagatatc tccattaaca tataccattc tgaaactaaa gacatagaca
181atcccccaag aaatgaaaca actgaaagta ctgaaaaaat gtacaaaatg tcaactatga
241gacgaatatt cgatttggca aagcatcgaa caaaaagatc cgcatttttc ccaacggggg
301ttaaagtctg tccacaggaa tccatgaaac agattttaga cagtcttcaa gcttattata
361gattgagagt gtgtcaggaa gcagtatggg aagcatatcg gatctttctg gatcgcatcc
421ctgacacagg ggaatatcag gactgggtca gcatctgcca gcaggagacc ttctgcctct
481ttgacattgg aaaaaacttc agcaattccc aggagcacct ggatcttctc cagcagagaa
541taaaacagag aagtttccct gacagaaaag atgaaatatc tgcagagaag acattgggag
601agcctggtga aaccattgtc atttcaacag atgttgccaa cgtctcactt gggcctttcc
661ctctcactcc tgatgacacc ctcctcaatg aaattctcga taatacactc aacgacacca
721agatgcctac aacagaaaga gaaacagaat tcgctgtgtt ggaggagcag agggtggagc
781tcagcgtctc tctggtaaac cagaagttca aggcagagct cgctgactcc cagtccccat
841attaccagga gctagcagga aagtcccaac ttcagatgca aaagatattt aagaaacttc
901caggattcaa aaaaatccat gtgttaggat ttagaccaaa gaaagaaaaa gatggctcaa
961gctccacaga gatgcaactt acggccatct ttaagagaca cagtgcagaa gcaaaaagcc
1021ctgcaagtga cctcctgtct tttgattcca acaaaattga aagtgaggaa gtctatcatg
1081gaaccatgga ggaggacaag caaccagaaa tctatctcac agctacagac ctcaaaaggc
1141tgatcagcaa agcactagag gaagaacaat ctttggatgt ggggacaatt cagttcactg
1201atgaaattgc tggatcactg ccagcctttg gtcctgacac ccaatcagag ctgcccacat
1261cttttgctgt tataacagag gatgctactt tgagtccaga acttcctcct gttgaacccc
1321agcttgagac agtggacgga gcagagcatg gtctacctga cacttcttgg tctccacctg
1381ctatggcctc tacctccctg tcagaagctc cacctttctt tatggcatca agcatcttct
1441ctctgactga tcaaggcacc acagatacaa tggccactga ccagacaatg ctagtaccag
1501ggctcaccat ccccaccagt gattattctg caatcagcca actggctctg ggaatttcac
1561atccacctgc atcttcagat gacagccgat caagtgcagg tggcgaagat atggtcagag
1621acctagatga aatggatctg tctgacactc ctgccccatc tgaggtacca gagctcagcg
1681aatatgtttc tgtcccagat catttcttgg aggataccac tcctgtctca gctttacagt
1741atatcaccac tagttctatg accattgccc ccaagggccg agagctggta gtgttcttca
1801gtctgcgtgt tgctaacatg gccttctcca acgacctgtt caacaagagc tctctggagt
1861accgagctct ggagcaacaa ttcacacagc tgctggttcc atatctacga tccaatctta
1921caggatttaa gcaacttgaa atacttaact tcagaaacgg gagtgtgatt gtgaatagca
1981aaatgaagtt tgctaagtca gtgccgtata acctcaccaa ggctgtgcac ggggtcttgg
2041aggattttcg ttctgctgca gcccaacaac tccatctgga aatagacagc tactctctca
2101acattgaacc agctgatcaa gcagatccct gcaagttcct ggcctgcggc gaatttgccc
2161aatgtgtaaa gaacgaatgg actgaggaag cggagtgtcg ctgcaaacca ggatatgaca
2221gccaggggag cctggacggt ctggaaccag gcctctgtgg ccctggcaca aaggaatgcg
2281aggtcctcca gggaaaggga gctccatgca ggttgccaga tcactctgaa aatcaagcat
2341acaaaactag tgttaaaaag ttccaaaatc aacaaaataa caaggtaatc agtaaaagaa
2401attctgaatt actgaccgta gaatatgaag aatttaacca tcaagattgg gaaggaaatt
2461tgccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg
2521attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct
2581ggatta

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