Construct: ORF ccsbBroad304_06452
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009329.1_s304c1, BRDN0000405257
- Derived from:
- ccsbBroadEn_06452
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IMPG1 (3617)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06452
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3617 | IMPG1 | interphotoreceptor matrix p... | NM_001563.4 | 99.9% | 99.7% | 1552C>G;2110C>T |
| 2 | human | 3617 | IMPG1 | interphotoreceptor matrix p... | NM_001282368.1 | 90.1% | 89.9% | 65_66ins234;1318C>G;1876C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2460
- ORF length:
- 2391
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtatttggaa actagaagag ctatttttgt tttttggatt tttctccaag 121 ttcaaggaac caaagatatc tccattaaca tataccattc tgaaactaaa gacatagaca 181 atcccccaag aaatgaaaca actgaaagta ctgaaaaaat gtacaaaatg tcaactatga 241 gacgaatatt cgatttggca aagcatcgaa caaaaagatc cgcatttttc ccaacggggg 301 ttaaagtctg tccacaggaa tccatgaaac agattttaga cagtcttcaa gcttattata 361 gattgagagt gtgtcaggaa gcagtatggg aagcatatcg gatctttctg gatcgcatcc 421 ctgacacagg ggaatatcag gactgggtca gcatctgcca gcaggagacc ttctgcctct 481 ttgacattgg aaaaaacttc agcaattccc aggagcacct ggatcttctc cagcagagaa 541 taaaacagag aagtttccct gacagaaaag atgaaatatc tgcagagaag acattgggag 601 agcctggtga aaccattgtc atttcaacag atgttgccaa cgtctcactt gggcctttcc 661 ctctcactcc tgatgacacc ctcctcaatg aaattctcga taatacactc aacgacacca 721 agatgcctac aacagaaaga gaaacagaat tcgctgtgtt ggaggagcag agggtggagc 781 tcagcgtctc tctggtaaac cagaagttca aggcagagct cgctgactcc cagtccccat 841 attaccagga gctagcagga aagtcccaac ttcagatgca aaagatattt aagaaacttc 901 caggattcaa aaaaatccat gtgttaggat ttagaccaaa gaaagaaaaa gatggctcaa 961 gctccacaga gatgcaactt acggccatct ttaagagaca cagtgcagaa gcaaaaagcc 1021 ctgcaagtga cctcctgtct tttgattcca acaaaattga aagtgaggaa gtctatcatg 1081 gaaccatgga ggaggacaag caaccagaaa tctatctcac agctacagac ctcaaaaggc 1141 tgatcagcaa agcactagag gaagaacaat ctttggatgt ggggacaatt cagttcactg 1201 atgaaattgc tggatcactg ccagcctttg gtcctgacac ccaatcagag ctgcccacat 1261 cttttgctgt tataacagag gatgctactt tgagtccaga acttcctcct gttgaacccc 1321 agcttgagac agtggacgga gcagagcatg gtctacctga cacttcttgg tctccacctg 1381 ctatggcctc tacctccctg tcagaagctc cacctttctt tatggcatca agcatcttct 1441 ctctgactga tcaaggcacc acagatacaa tggccactga ccagacaatg ctagtaccag 1501 ggctcaccat ccccaccagt gattattctg caatcagcca actggctctg ggaatttcac 1561 atccacctgc atcttcagat gacagccgat caagtgcagg tggcgaagat atggtcagag 1621 acctagatga aatggatctg tctgacactc ctgccccatc tgaggtacca gagctcagcg 1681 aatatgtttc tgtcccagat catttcttgg aggataccac tcctgtctca gctttacagt 1741 atatcaccac tagttctatg accattgccc ccaagggccg agagctggta gtgttcttca 1801 gtctgcgtgt tgctaacatg gccttctcca acgacctgtt caacaagagc tctctggagt 1861 accgagctct ggagcaacaa ttcacacagc tgctggttcc atatctacga tccaatctta 1921 caggatttaa gcaacttgaa atacttaact tcagaaacgg gagtgtgatt gtgaatagca 1981 aaatgaagtt tgctaagtca gtgccgtata acctcaccaa ggctgtgcac ggggtcttgg 2041 aggattttcg ttctgctgca gcccaacaac tccatctgga aatagacagc tactctctca 2101 acattgaacc agctgatcaa gcagatccct gcaagttcct ggcctgcggc gaatttgccc 2161 aatgtgtaaa gaacgaatgg actgaggaag cggagtgtcg ctgcaaacca ggatatgaca 2221 gccaggggag cctggacggt ctggaaccag gcctctgtgg ccctggcaca aaggaatgcg 2281 aggtcctcca gggaaaggga gctccatgca ggttgccaga tcactctgaa aatcaagcat 2341 acaaaactag tgttaaaaag ttccaaaatc aacaaaataa caaggtaatc agtaaaagaa 2401 attctgaatt actgaccgta gaatatgaag aatttaacca tcaagattgg gaaggaaatt 2461 tgccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 2521 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 2581 ggatta