Construct: ORF ccsbBroad304_06453
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010990.1_s304c1, BRDN0000411129
- Derived from:
- ccsbBroadEn_06453
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ING2 (3622)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06453
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3622 | ING2 | inhibitor of growth family ... | NM_001564.4 | 99.8% | 100% | 39C>T |
| 2 | human | 3622 | ING2 | inhibitor of growth family ... | NM_001291959.1 | 83.9% | 80.3% | (many diffs) |
| 3 | human | 3622 | ING2 | inhibitor of growth family ... | XM_011531927.2 | 80% | 79.6% | 0_1ins166;3G>C;6delT |
| 4 | mouse | 69260 | Ing2 | inhibitor of growth family,... | NM_023503.3 | 90.1% | 96.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 906
- ORF length:
- 840
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtt agggcagcag cagcagcaac tgtactcgtc ggctgcgctc ctgaccgggg 121 agcggagccg gctgctcacc tgctacgtgc aggactacct tgagtgcgtg gagtcgctgc 181 cccacgacat gcagaggaac gtgtctgtgc tgcgagagct ggacaacaaa tatcaagaaa 241 cgttaaagga aattgatgat gtctacgaaa aatataagaa agaagatgat ttaaaccaga 301 agaaacgtct acagcagctt ctccagagag cactaattaa tagtcaagaa ttgggagatg 361 aaaaaataca gattgttaca caaatgctcg aattggtgga aaatcgggca agacaaatgg 421 agttacactc acagtgtttc caagatcctg ctgaaagtga acgagcctca gataaagcaa 481 agatggattc cagccaacca gaaagatctt caagaagacc ccgcaggcag cggaccagtg 541 aaagccgtga tttatgtcac atggcaaatg ggattgaaga ctgtgatgat cagccaccta 601 aagaaaagaa atccaagtca gcaaagaaaa agaaacgctc caaggccaag caggaaaggg 661 aagcttcacc tgttgagttt gcaatagatc ctaatgaacc tacatactgc ttatgcaacc 721 aagtgtctta tggggagatg ataggatgtg acaatgaaca gtgtccaatt gaatggtttc 781 acttttcatg tgtttcactt acctataaac caaaggggaa atggtattgc ccaaagtgca 841 ggggagataa tgagaaaaca atggacaaaa gtactgaaaa gacaaaaaag gatagaagat 901 cgaggtaccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 961 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 1021 acctctggat ta