Construct: ORF ccsbBroad304_06464
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010942.1_s304c1, BRDN0000409304
- Derived from:
- ccsbBroadEn_06464
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ITGB2 (3689)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06464
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3689 | ITGB2 | integrin subunit beta 2 | NM_000211.5 | 99.8% | 100% | 24G>T;1101C>A;1323T>C |
| 2 | human | 3689 | ITGB2 | integrin subunit beta 2 | NM_001127491.3 | 99.8% | 100% | 24G>T;1101C>A;1323T>C |
| 3 | human | 3689 | ITGB2 | integrin subunit beta 2 | NM_001303238.2 | 90.9% | 91% | 0_1ins207;894C>A;1116T>C |
| 4 | human | 3689 | ITGB2 | integrin subunit beta 2 | XM_006724001.2 | 90.9% | 91% | 0_1ins207;894C>A;1116T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2373
- ORF length:
- 2307
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct gggcctgcgc cccccacttc tcgccctggt ggggctgctc tccctcgggt 121 gcgtcctctc tcaggagtgc acgaagttca aggtcagcag ctgccgggaa tgcatcgagt 181 cggggcccgg ctgcacctgg tgccagaagc tgaacttcac agggccgggg gatcctgact 241 ccattcgctg cgacacccgg ccacagctgc tcatgagggg ctgtgcggct gacgacatca 301 tggaccccac aagcctcgct gaaacccagg aagaccacaa tgggggccag aagcagctgt 361 ccccacaaaa agtgacgctt tacctgcgac caggccaggc agcagcgttc aacgtgacct 421 tccggcgggc caagggctac cccatcgacc tgtactatct gatggacctc tcctactcca 481 tgcttgatga cctcaggaat gtcaagaagc taggtggcga cctgctccgg gccctcaacg 541 agatcaccga gtccggccgc attggcttcg ggtccttcgt ggacaagacc gtgctgccgt 601 tcgtgaacac gcaccctgat aagctgcgaa acccatgccc caacaaggag aaagagtgcc 661 agcccccgtt tgccttcagg cacgtgctga agctgaccaa caactccaac cagtttcaga 721 ccgaggtcgg gaagcagctg atttccggaa acctggatgc acccgagggt gggctggacg 781 ccatgatgca ggtcgccgcc tgcccggagg aaatcggctg gcgcaacgtc acgcggctgc 841 tggtgtttgc cactgatgac ggcttccatt tcgcgggcga cgggaagctg ggcgccatcc 901 tgacccccaa cgacggccgc tgtcacctgg aggacaactt gtacaagagg agcaacgaat 961 tcgactaccc atcggtgggc cagctggcgc acaagctggc tgaaaacaac atccagccca 1021 tcttcgcggt gaccagtagg atggtgaaga cctacgagaa actcaccgag atcatcccca 1081 agtcagccgt gggggagctg tctgaggact ccagcaatgt ggtccatctc attaagaatg 1141 cttacaataa actctcctcc agggtattcc tggatcacaa cgccctcccc gacaccctga 1201 aagtcaccta cgactccttc tgcagcaatg gagtgacgca caggaaccag cccagaggtg 1261 actgtgatgg cgtgcagatc aatgtcccga tcaccttcca ggtgaaggtc acggccacag 1321 agtgcatcca ggagcagtcg tttgtcatcc gggcgctggg cttcacggac atagtgaccg 1381 tgcaggtcct tccccagtgt gagtgccggt gccgggacca gagcagagac cgcagcctct 1441 gccatggcaa gggcttcttg gagtgcggca tctgcaggtg tgacactggc tacattggga 1501 aaaactgtga gtgccagaca cagggccgga gcagccagga gctggaagga agctgccgga 1561 aggacaacaa ctccatcatc tgctcagggc tgggggactg tgtctgcggg cagtgcctgt 1621 gccacaccag cgacgtcccc ggcaagctga tatacgggca gtactgcgag tgtgacacca 1681 tcaactgtga gcgctacaac ggccaggtct gcggcggccc ggggaggggg ctctgcttct 1741 gcgggaagtg ccgctgccac ccgggctttg agggctcagc gtgccagtgc gagaggacca 1801 ctgagggctg cctgaacccg cggcgtgttg agtgtagtgg tcgtggccgg tgccgctgca 1861 acgtatgcga gtgccattca ggctaccagc tgcctctgtg ccaggagtgc cccggctgcc 1921 cctcaccctg tggcaagtac atctcctgcg ccgagtgcct gaagttcgaa aagggcccct 1981 ttgggaagaa ctgcagcgcg gcgtgtccgg gcctgcagct gtcgaacaac cccgtgaagg 2041 gcaggacctg caaggagagg gactcagagg gctgctgggt ggcctacacg ctggagcagc 2101 aggacgggat ggaccgctac ctcatctatg tggatgagag ccgagagtgt gtggcaggcc 2161 ccaacatcgc cgccatcgtc gggggcaccg tggcaggcat cgtgctgatc ggcattctcc 2221 tgctggtcat ctggaaggct ctgatccacc tgagcgacct ccgggagtac aggcgctttg 2281 agaaggagaa gctcaagtcc cagtggaaca atgataatcc ccttttcaag agcgccacca 2341 cgacggtcat gaaccccaag tttgctgaga gttacccaac tttcttgtac aaagtggttg 2401 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 2461 accggtggcg cgttaagtcg acaatcaacc tctggatta