Construct: ORF ccsbBroad304_06563
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016959.1_s304c1, BRDN0000462186
- Derived from:
- ccsbBroadEn_06563
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MATN2 (4147)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06563
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4147 | MATN2 | matrilin 2 | NM_030583.4 | 99.7% | 99.8% | (many diffs) |
| 2 | human | 4147 | MATN2 | matrilin 2 | NM_002380.5 | 97.7% | 97.9% | (many diffs) |
| 3 | human | 4147 | MATN2 | matrilin 2 | XM_017013417.2 | 95.4% | 95.5% | (many diffs) |
| 4 | human | 4147 | MATN2 | matrilin 2 | NM_001317748.2 | 93.5% | 93.6% | (many diffs) |
| 5 | human | 4147 | MATN2 | matrilin 2 | XM_017013418.2 | 85% | 85% | (many diffs) |
| 6 | human | 4147 | MATN2 | matrilin 2 | XM_005250920.2 | 83.3% | 83.3% | (many diffs) |
| 7 | mouse | 17181 | Matn2 | matrilin 2 | NM_016762.2 | 86.2% | 87.3% | (many diffs) |
| 8 | mouse | 17181 | Matn2 | matrilin 2 | XM_006520023.2 | 84.5% | 85.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2877
- ORF length:
- 2811
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga aaagatgctc gcaggctgct ttctgctgat cctcggacag atcgtcctcc 121 tccctgccga ggccagggag cggtcacgtg ggaggtccat ctctaggggc agacacgctc 181 ggacccaccc gcagacggcc cttctggaga gttcctgtga gaacaagcgg gcagacctgg 241 ttttcatcat tgacagctct cgcagtgtca acacccatga ctatgcaaag gtcaaggagt 301 tcatcgtgga catcttgcaa ttcttggaca ttggtcctga tgtcacccga gtgggcctgc 361 tccaatatgg cagcactgtc aagaatgagt tctccctcaa gaccttcaag aggaagtccg 421 aggtggagcg tgctgtcaag aggatgcggc atctgtccac gggcaccatg accgggctgg 481 ccatccagta tgccctgaac atcgcattct cagaagcaga gggggcccgg cccctgaggg 541 agaatgtgcc acgggtcata atgatcgtga cagatgggag acctcaggac tccgtggccg 601 aggtggctgc taaggcacgg gacacgggca tcctaatctt tgccattggt gtgggccagg 661 tagacttcaa caccttgaag tccattggga gtgagcccca tgaggaccat gtcttccttg 721 tggccaattt cagccagatt gagacgctga cctccgtgtt ccagaagaag ttgtgcacgg 781 cccacatgtg cagcaccctg gagcataact gtgcccactt ctgcatcaac atccctggct 841 catacgtctg caggtgcaaa caaggctaca ttctcaactc ggatcagacg acttgcagaa 901 tccaggatct gtgtgccatg gaggaccaca actgtgagca gctctgtgtg aatgtgccgg 961 gctccttcgt ctgccagtgc tacagtggct acgccctggc tgaggatggg aagaggtgtg 1021 tggctgtgga ctactgtgcc tcagaaaacc acggatgtga acatgagtgt gtaaatgctg 1081 atggctccta cctttgccag tgccatgaag gatttgctct taacccagat gaaaaaacgt 1141 gcacaaagat agactactgt gcctcatcta atcatggatg tcagcatgag tgtgttaaca 1201 cagatgattc ctattcctgc cactgcctga aaggctttac cctgaatcca gataagaaaa 1261 cctgcagaag gatcaactac tgtgcactga acaaaccggg ctgtgagcat gagtgcgtca 1321 acatggagga gagctactac tgccgctgcc accgtggcta cactctggac cccaatggca 1381 aaacctgcag ccgagtggac cactgtgcac agcaggacca tggctgtgag cagctgtgtc 1441 tgaacacgga ggattccttc gtctgccagt gctcagaagg cttcctcatc aacgaggacc 1501 tcaagacctg ctcccgggtg gattactgcc tgctgagtga ccatggttgt gaatactcct 1561 gtgtcaacat ggacagatcc tttgcctgtc agtgtcctga gggacacgtg ctccgcagcg 1621 atgggaagac gtgtgcaaaa ttggactctt gtgctctggg ggaccacggt tgtgaacatt 1681 cgtgtgtaag cagtgaagat tcgtttgtgt gccagtgctt tgaaggttat atactccgtg 1741 aagatggaaa aacctgcaga aggaaagatg tctgccaagc tatagaccat ggctgtgaac 1801 acatttgtgt gaacagtgac gactcataca cgtgcgagtg cttggaggga ttccggctcg 1861 ctgaggatgg gaaacgctgc cgaaggaagg atgtctgcaa atcaacccac catggctgcg 1921 aacacatttg tgttaataat gggaattcct acatctgcaa atgctcagag ggatttgttc 1981 tagctgagga cggaagacgg tgcaagaaat gcactgaagg cccaattgac ctggtctttg 2041 tgatcgatgg atccaagagt cttggagaag agaattttga ggtcgtgaag cagtttgtca 2101 ctggaattat agattccttg acaatttccc ccaaagccgc tcgagtgggg ctgctccagt 2161 attccacaca ggtccacaca gagttcaccc tgagaaactt caactcagcc aaagacatga 2221 aaaaagccgt ggcccacatg aaatacatgg gaaagggctc tatgactggg ctggccctga 2281 aacacatgtt tgagagaagt tttacccaag gagaaggggc caggcccctt tccacaaggg 2341 tgcccagagc agccattgtg ttcaccgacg gacgggctca ggatgacgtc tccgagtggg 2401 ccagtaaagc caaggccaat ggtatcacta tgtatgctgt tggggtagga aaagccattg 2461 aggaggaact acaagagatt gcctctgagc ccacaaacaa gcatctcttc tatgccgaag 2521 acttcagcac aatggatgag ataagtgaaa aactcaagaa aggcatctgt gaagctctag 2581 aagactccga tggaagacag gactctccag caggggaact gccaaaaacg gtccaacagc 2641 caacagtgca acacagatat ctgtttgaag aagacaatct tttacggtct acacaaaagc 2701 tttcccattc aacaaaacct tcaggaagcc ctttggaaga aaaacacgat caatgcaaat 2761 gtgaaaacct tataatgttc cagaaccttg caaacgaaga agtaagaaaa ttaacacagc 2821 gcttagaaga aatgacacag agaatggaag ccctggaaaa tcgcctgaga tacagatgcc 2881 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 2941 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 3001 tta